Recombinant
RabMAb

Recombinant Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

Overview

  • Product name

    Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free
    See all Brachyury / Bry primary antibodies
  • Description

    Rabbit monoclonal [EPR18113] to Brachyury / Bry - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC - Wholemount, IP, ICC/IF, IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Brachyury/ Bry aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: O15178

  • Positive control

    • IHC-P: Mouse E14.5 embryo tissue.
  • General notes

    Ab236023 is the carrier-free version of ab209665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236023 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.

Target

  • Function

    Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • Involvement in disease

    Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Images

  • Ab209665 staining Brachyury/Bry in MUG-Chor1 by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a 1/1000 dilution.  An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at a 1/1000 dilution.  An Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain antibody.  DAPI was used as nuclear counterstain. Nuclear staining on MUG-Chor1 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • IHC - Wholemount of E6.5 wholemount mouse embryo labelling Brachyury / Bry with ab209665. Sample was incubated with primary antibody (1/100 in PBS/0.1% Triton-X/5% donkey serum/1% BSA) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG monoclonal was used as the secondary antibody (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    See Abreview

  • Lane 1 (input):  MUG-Chor1 (human sacral bone chordoma) whole cell lysate, 10 μg
    Lane 2 (+): MUG-Chor1 whole cell lysate
    Lane 3 (-):  Rabbit monoclonal IgG (ab172730) instead of  ab209665 in MUG-Chor1 whole cell lysate

    Ab209665 immunoprecipitating Brachyury/Bry in MUG-Chor1 lysates. For western blotting, primary antibody used was ab209665 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking and diluting buffer used was 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Ab209665 staining Brachyury/Bry in MUG-Chor1 (Human sacral bone chordoma) cell line by Flow cytometry. Cells were fixed with 4% paraformaldehyde and 90% methanol. Sample was incubated with primary antibody at 1/400 dilution (red). A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution.  Rabbit monoclonal IgG (ab172730) (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Ab209665 staining Brachyury/Bry in Rat E14.5 embryo tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed paraffin and antigen retrieval was performed by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/4000 dilution. A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter-stain. Nuclear staining on notochord of rat E14.5 embryo.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunohistochemical analysis of paraffin-embedded Human chordoma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human chordoma is observed. Counter stained with Hematoxylin

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunohistochemical analysis of paraffin-embedded Human meningioma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human meningioma. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunohistochemical analysis of paraffin-embedded Human chondrosarcoma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human chondrosarcoma. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • ab209665 staining Brachyury/Bry in mouse E14.5 embryo tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed paraffin and antigen retrieval was performed by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/4000 dilution. A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter-stain. Nuclear staining on notochord of mouse E14.5 embryo.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

References

ab236023 has not yet been referenced specifically in any publications.

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