Overview

  • Product name

    Bradford Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    0h 20m
  • Product overview

    Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.


    The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.


    Bradford assay protocol summary:
    - prepare serial dilutions of protein standard
    - add samples and standards to wells or tubes
    - add Bradford protein assay solution and incubate for 5 min at room temp
    - measure absorbance at 595 nm

  • Notes

    This product was previously called Protein Quantitation Kit (Bradford Assay).

  • Platform

    Microplate reader

Properties

Images

  • Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.

  • Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted

Protocols

References

This product has been referenced in:

  • Gan SY  et al. Fucosterol exerts protection against amyloid ß-induced neurotoxicity, reduces intracellular levels of amyloid ß and enhances the mRNA expression of neuroglobin in amyloid ß-induced SH-SY5Y cells. Int J Biol Macromol 121:207-213 (2019). Read more (PubMed: 30300695) »
  • Greish K  et al. Synthetic cannabinoids nano-micelles for the management of triple negative breast cancer. J Control Release 291:184-195 (2018). Read more (PubMed: 30367922) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I can confirm that both BCA and Bradford protein quantification kits can be used for human serum and plasma samples. The main difference between the two kits is that the BCA assay uses a Copper ion based chemistry whereas the Bradford assay uses a Coomassie-dye based reaction to produce color. The addition of heparin on your sample can form heparin-copper complex that can chelate out the copper ions in the BCA reagent. Therefore, in your particular case I will suggest to use the Bradford Assay (ab102535).

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Question
Answer

Thank you for contacting us and your interest in our products.

The two kits you have chosen should be suitable alternatives for the customer wanting to quantify protein in their samples.

You may also want to bring the BCA Protein Quantification Kit (ab102536) to their attention. This kit also allows the quantification of protein but using an alternative reagent to Bradford.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

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