Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts
Product nameBradford Assay Kit
Sample typeSerum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
Assay time0h 20m
Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.
The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
Bradford assay protocol summary:
- prepare serial dilutions of protein standard
- add samples and standards to wells or tubes
- add Bradford protein assay solution and incubate for 5 min at room temp
- measure absorbance at 595 nm
Support on how to determine protein concentration can be found here
This product is manufactured by BioVision, an Abcam company and was previously called K810 Protein Quantitation Kit. K810-1000 is the same size as the 1000 test size of ab102535.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1000 tests Protein Assay Solution NM 1 x 20ml Protein Standard Yellow 1 x 1.5ml
RelevanceProtein Quantitation is a procedure for determining the concentration of protein in solution.
Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.
Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted.
Colorimetric standard curve of 10 microliter samples: mean of duplicates with background readings subtracted.
ab102535 has been referenced in 16 publications.
- Li S et al. NLRX1/FUNDC1/NIPSNAP1-2 axis regulates mitophagy and alleviates intestinal ischaemia/reperfusion injury. Cell Prolif 54:e12986 (2021). PubMed: 33432610
- Maunder E et al. Temperate performance and metabolic adaptations following endurance training performed under environmental heat stress. Physiol Rep 9:e14849 (2021). PubMed: 33977674
- Guillory RJ et al. Zn2+-dependent suppression of vascular smooth muscle intimal hyperplasia from biodegradable zinc implants. Mater Sci Eng C Mater Biol Appl 111:110826 (2020). PubMed: 32279804
- El-Garawani I et al. Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae. Pharmaceutics 12:N/A (2020). PubMed: 33227988
- Guillory RJ et al. Preclinical In-Vivo Evaluation and Screening of Zinc Based Degradable Metals for Endovascular Stents. JOM (1989) 71:1436-1446 (2019). PubMed: 33731979