Overview

  • Product name

    Bradford Reagent
  • General notes

    Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.

    This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.

    When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.

    Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443

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Customer reviews and Q&As

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Question
Answer

Thank you for contacting us and your interest in our products.

The two kits you have chosen should be suitable alternatives for the customer wanting to quantify protein in their samples.

You may also want to bring the BCA Protein Quantification Kit (ab102536) to their attention. This kit also allows the quantification of protein but using an alternative reagent to Bradford.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

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Answer

Thank you for your email. Click on the link to check new range of ELISA kits; https://www.abcam.com/index.html?c=4398 Optiblot westernblot products https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot We wish you best of luck. Please do not hesitate to contact us for any additional questions or inquiries.

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