Product nameBradford Reagent
This product is manufactured by Expedeon, an Abcam company. Expedeon product code BFU05L was previously called Bradford Ultra - 500 ml and is the same as the 500 ml size of this product.
Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.
This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.
Storage instructionsShipped at Room Temperature. Store at +4°C.
Storage bufferConstituents: 5% Ethanol, 0.1% Coomassie Brilliant Blue, 12% Phosphoric acid
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This product has been referenced in:
- Matsuishi YI et al. Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1. Biochem Biophys Res Commun 495:1655-1660 (2018). Read more (PubMed: 29223396) »
- Lai YC et al. Aberrant expression of microRNAs and the miR-1/MET pathway in canine hepatocellular carcinoma. Vet Comp Oncol 16:288-296 (2018). Read more (PubMed: 29314614) »