Bradford Reagent (ab119216)
Overview
-
Product name
Bradford Reagent -
General notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.
Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.
This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.Support on how to determine protein concentration can be found here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at +4°C. -
Storage buffer
Constituents: 3% Ethanol, 2.3% α-Cyclodextrin hydrate, 0.23% gamma-Cyclodextrin, 14% Phosphoric acid -
Concentration information loading... -
Research areas
Images
-
Bradford Reagent (ab119216)
-
Bradford Reagent (ab119216) -
Bradford Reagent (ab119216) -
Bradford Reagent (ab119216)
Datasheets and documents
-
SDS download
-
Datasheet download
References (28)
ab119216 has been referenced in 28 publications.
- Matsuishi YI et al. Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1. Biochem Biophys Res Commun 495:1655-1660 (2018). PubMed: 29223396
- Lai YC et al. Aberrant expression of microRNAs and the miR-1/MET pathway in canine hepatocellular carcinoma. Vet Comp Oncol 16:288-296 (2018). PubMed: 29314614
- Ando F et al. AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus. Nat Commun 9:1411 (2018). PubMed: 29650969
- Ciesielska A et al. Bis(monoacylglycero)phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol 83:15-26 (2017). PubMed: 27939812
- Lyczakowski JJ et al. Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy. Biotechnol Biofuels 10:224 (2017). PubMed: 28932265