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Bradford Reagent (ab119216)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (28)

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Bradford Reagent (ab119216)
  • Bradford Reagent (ab119216)
  • Bradford Reagent (ab119216)
  • Bradford Reagent (ab119216)

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Preparation
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Nuclear Extraction Kit (ab113474)

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Overview

  • Product name

    Bradford Reagent
  • General notes

    This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.

    Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.

    This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.

    When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.

    Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.

    Support on how to determine protein concentration can be found here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at +4°C.
  • Storage buffer

    Constituents: 3% Ethanol, 14% Phosphoric acid, 2.3% α-Cyclodextrin hydrate, 0.23% gamma-Cyclodextrin
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein quantification kits
    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Protein Quantitation Kits
    • Bradford

Associated products

    Images

    • Bradford Reagent (ab119216)
      Bradford Reagent (ab119216)
    • Bradford Reagent (ab119216)
      Bradford Reagent (ab119216)
    • Bradford Reagent (ab119216)
      Bradford Reagent (ab119216)
    • Bradford Reagent (ab119216)
      Bradford Reagent (ab119216)

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (28)

    Publishing research using ab119216? Please let us know so that we can cite the reference in this datasheet.

    ab119216 has been referenced in 28 publications.

    • Matsuishi YI  et al. Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1. Biochem Biophys Res Commun 495:1655-1660 (2018). PubMed: 29223396
    • Lai YC  et al. Aberrant expression of microRNAs and the miR-1/MET pathway in canine hepatocellular carcinoma. Vet Comp Oncol 16:288-296 (2018). PubMed: 29314614
    • Ando F  et al. AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus. Nat Commun 9:1411 (2018). PubMed: 29650969
    • Ciesielska A  et al. Bis(monoacylglycero)phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol 83:15-26 (2017). PubMed: 27939812
    • Lyczakowski JJ  et al. Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy. Biotechnol Biofuels 10:224 (2017). PubMed: 28932265
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Can I ask customer to use ab102535 or ab119216 against the below queries ?

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 11 2012

    Answer

    Thank you for contacting us and your interest in our products.

    The two kits you have chosen should be suitable alternatives for the customer wanting to quantify protein in their samples.

    You may also want to bring the BCA Protein Quantification Kit (ab102536) to their attention. This kit also allows the quantification of protein but using an alternative reagent to Bradford.

    I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Dec 11 2012

    Question

    Thank you. The information you provided was very useful. I have sent a request for quotation. Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2012

    Answer

    Thank you for your email. Click on the link to check new range of ELISA kits; https://www.abcam.com/index.html?c=4398 Optiblot westernblot products https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot We wish you best of luck. Please do not hesitate to contact us for any additional questions or inquiries.

    Read More

    Abcam Scientific Support

    Answered on Jan 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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