Key features and details
- Sensitivity: 24.8 pg/ml
- Range: 11.7 pg/ml - 30000 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
Product nameBradykinin ELISA kit
Intra-assay Sample n Mean SD CV% Buffer 20 695.4pg/ml 4.6% Buffer 20 208.7pg/ml 6.2% Buffer 20 73.7pg/ml 9.9% Inter-assay Sample n Mean SD CV% Buffer 700.4pg/ml = 15% Buffer 209.3pg/ml = 10.3% Buffer 66.1pg/ml = 11.9%
Sample typeUrine, Serum, Plasma
Range11.7 pg/ml - 30000 pg/ml
Sample specific recovery Sample type Average % Range Urine 109 10pg/ml - 2000pg/ml Serum 113 100pg/ml - 2000pg/ml Plasma 110 100pg/ml - 20000pg/ml
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Species independent
Bradykinin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the accurate quantitative measurement of Bradykinin in plasma, serum and urine.
A goat anti-Rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of Bradykinin conjugated to biotin, followed by a solution of polyclonal antibody to Bradykinin. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of Bradykinin captured in the plate.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 16 1 x 55ml Bradykinin Antibody 1 x 5ml Bradykinin Conjugate 1 x 5ml Bradykinin Standard 2 vials Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit HRP- Streptavidin Conjugate 1 x 12.5µg Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 2 x 10ml
Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
Sequence similaritiesContains 3 cystatin domains.
modificationsBradykinin is released from kininogen by plasma kallikrein.
Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
Phosphorylation sites are present in the extracelllular medium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- Alpha-2-thiol proteinase inhibitor
- Fitzgerald factor
ab136936 has been referenced in 3 publications.
- Matsuura H et al. C1 Esterase Inhibitor Activity Is Reduced in the Acute Phase Following Burn Injury: A Prospective Observational Study. J Burn Care Res 40:893-899 (2019). PubMed: 31250897
- Ito M et al. Prostanoid-dependent spontaneous pain and PAR2-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4. Mol Pain 13:1744806917704138 (2017). PubMed: 28381109
- Vieira ML et al. Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. PLoS Negl Trop Dis 10:e0004713 (2016). PubMed: 27167223