Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-B Raf antibody [EP152Y]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult brain)
Specification
adult brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid Hier, pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
A highly different pattern of immunolabelling /staining using ab33899 compared with ab65050.
The highest level of immunopositivity is restricted to Dendritic fields. I observe only a very low level of neuronal cytoplasmic positivity throughout the mouse brain sagittal section that I used.
Not recommended for paraffin wax IHC of mouse, in my opinion.
I applied the same IHC variables as ab65050, to attempt to achieve more convincing results....no success.
My apologies for the artefactual "shattering" observed in the upper image: this is due to inadequate care when sectioning pwax sections.
Abcam response
Thank you for your honest and thoughtful feedback. I am sorry this antibody is not working to expectation. Under the terms of our Abpromise, we would be happy to offer a replacement or refund for this antibody.

Mr. Carl Hobbs

Verified customer

Submitted Feb 02 2009

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