BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Non-Abcam antibody was used: anti rabbit Igs Host species: Goat Clonality: Polyclonal Conjugation: Biotin
A highly different pattern of immunolabelling /staining using ab33899 compared with ab65050. The highest level of immunopositivity is restricted to Dendritic fields. I observe only a very low level of neuronal cytoplasmic positivity throughout the mouse brain sagittal section that I used. Not recommended for paraffin wax IHC of mouse, in my opinion. I applied the same IHC variables as ab65050, to attempt to achieve more convincing results....no success. My apologies for the artefactual "shattering" observed in the upper image: this is due to inadequate care when sectioning pwax sections.
Thank you for your honest and thoughtful feedback. I am sorry this antibody is not working to expectation. Under the terms of our Abpromise, we would be happy to offer a replacement or refund for this antibody.
Mr. Carl Hobbs
Submitted Feb 02 2009
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