Western blot abreview for Anti-B Raf antibody [EP152Y]

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma cell lines)
Loading amount
50 µg
Specification
melanoma cell lines
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS,0.02% Tween

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 87 kDa

Additional data

Additional Notes
weak but specific band for G361, stronger signal under reducing conditions

G361 and A2058 are human melanoma cell lines

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Submitted Jun 02 2010

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