brain, liver, skin from Tg animal expressing Human
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
16 hour(s) and 0 minute(s)
Non-Abcam antibody was used: Goat anti rabbit HRP Host species: Goat Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
5 minute(s) and 0 second(s)
Specific: 95 kDa
This antibody data sheet suggests it does not cross react with Mouse BRAF. But I ran three Wb using Human melanoma, Hela Cell line lysates and also used Wild Type mouse melanocytes, p53 -/- MEFs and various tissue lysates from mouse such as BRAIN, Liver, Lung, Kidney, Heart,and skin (from Transgenic mouse which has human BRAF). But this antibody reacts with both human and mouse BRAF. With Human it showed one band and for mouse it showed two bands because mouse BRAF undergoes alternate splicing.
Negative control various mouse lysate as mention above.
This anitbody reacts human and mouse very well infact mouse showed better signal than human
Thank you for your Abreview. Previous data suggested that the antibody did not cross-react with mouse. I am sure this information will be helpful to future customers.
Abcam user community
Submitted Oct 02 2006
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