Product nameAnti-BRAF (mutated V600 E) antibody [VE1]
See all BRAF primary antibodies
DescriptionMouse monoclonal [VE1] to BRAF (mutated V600 E)
The VE1 monoclonal is a sensitive antibody that detects mutated, constitutively active BRAF protein where glutamic acid is present at codon 600 instead of valine (V600E) (PubMed IDs: 21638088, 23657789).
Please be aware that non-specific nuclear staining has been reported with this antibody (PubMed IDs: 23763264, 23589031, 24838325).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken
Synthetic peptide within Human BRAF aa 550-650 (mutated V600 E). The exact sequence is proprietary.
Database link: P15056
- IHC-P: Human colon carcinoma tissue with B-RAF V600E mutation.
The V600E activating mutation in BRAF is found in several cancer types including: ~65% of pleomorphic xanthoastrocytomas, ~52% of microsatellite-unstable colon cancer tumors, ~50% of melanomas, ~35% of papillary thyroid carcinomas and ~5% of microsatellite-stable colon cancers (PubMed IDs: 21274720, 24508103, 18682506, 16024606).
The majority (>90%) of BRAF mutant cancers harbor the V600E mutation. The mutation leads to activation of the MAPK signaling pathway that increases cell invasion and reduces apoptosis. It also leads to reduced expression of melanocyte differentiation antigens and subsequent immune evasion (PubMed IDs: 21638088, 20551059).
The VE1 monoclonal antibody is manufactured by Abcam. If you require a particular buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab228461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Tissue specificityBrain and testis.
Involvement in diseaseNote=Defects in BRAF are found in a wide range of cancers.
Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Cellular localizationNucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
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BRAF V600E IHC positive PXA cases molecularly confirmed as BRAF V600E mutant tumors: strong granular cytoplasmic immunostaining of a characteristic pleomorphic multinucleated giant tumor cell (A), weak granular cytoplasmic immunostaining of pleomorphic and spindle tumor cells (B), strong granular cytoplasmic immunostaining of a cluster of isolated tumor cells (C).
Four-micron freshly cut sections (<2 weeks) of formalin-fixed, paraffin-embedded (FFPE) tissue from human were dried and melted at 62°C oven for 20 minutes. Subsequently, they were stained with ab228461. Staining was performed on the Ventana BenchMark XT (Ventana Medical Systems Inc.). The staining protocol included online deparaffinization, HIER (Heat Induced Epitope Retrieval) with Ventana Cell Conditioning 1 for 32 minutes and primary antibody incubation for 32 minutes at 37°C.
Representative photomicrographs serially stained for hematoxylin and eosin, PMS2 and BRAFV600E of (a) DNA mismatch repair-proficient/BRAF wild-type colorectal carcinoma and (b) DNA mismatch repair-proficient/BRAFV600E mutant tumor.
The slides were dewaxed in Bond Dewax solution and hydrated in Bond Wash solution. Heat-induced epitope retrieval was performed for 60 min in the manufacturer’s alkaline retrieval solution ER2. Slides were then incubated with the primary antibody at a dilution of 1 in 80 for 30 min at room temperature. Antibody detection was performed using the biotin-free Bond Polymer Defined Detection System. Slides were then counterstained with hematoxylin.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for B Raf (mutated V600E) using ab228461 at a 1/100 dilution in immunohistochemical analysis.
Samples from human tumor tissues that were Positive (A, E, I) and Negative (B, F, J) for BRAF expression. Boxes in A, E, I show the negative controls from their corresponding non-tumor tissues.
IHC for BRAF protein expression was performed on 4 μm-thick sections of formalin-fixed, paraffin-embedded tissues, using ab228461. The specimens were fixed in 10% neutral buffered formalin for 24–48 hours. after deparaffinization, the slides were pretreated with cell conditioning 1 for 64 minutes for antigen unmasking and followed by pre-primary antibody peroxidase inhibition. The slides were then incubated with the VE1 antibody at 37°C for 16 minutes, and counterstained with hematoxylin II for 4 minutes and bluing reagent for 4 minutes.
BRAF (mutated V600E) VE1 immunoreactivity in melanoma sections: (A) strongly positive homogeneous staining, (B) moderately positive homogeneous staining, (C) weakly positive homogeneous staining and (D) negative staining of a patients biopsy that was identified as BRAF (mutated V600D).
Sections were stained with undiluted ab228461. pre-treated with cell conditioner 1 (pH 8) for 60 min, followed by incubation with ab228461 at 37 °C for 32 min. Counterstained with one drop of haematoxylin for 4 min and one drop of bluing reagent for 4 min.
(A) Positive staining in a V600E2-mutated melanoma sample of patient 8 (homogenous brown colour indicating mutated cells) with ab228461 and (B) negative control.
BRAF V600E mutations in adamantinomatous craniopharyngioma. Two cases are shown (a–e and f–j). a, f Classical features of aCP (wet keratin, stellate reticulum, palisaded epithelium). b, g Translocation of β-catenin is shown in both cases (brown reaction product). c, h An antibody to BRAF V600E (VE1) shows staining of the tumour tissue (brown reaction product).
Immunohistochemical analysis was performed on 4µm sections from FFPE tumour specimens. Following dewaxing through graded alcohols, endogenous peroxidase activity was blocked (3% (v/v) H2O2 in PBS, pH 7.3, 20 minutes with orbital shaking). Epitope retrieval was achieved by autoclaving in sodium citrate (10mM, pH 6.0, 10 minutes) (β-catenin) or Tris-EDTA (10mM Tris base, 1mM EDTA, pH 9.0, 10 minutes) (BRAF). Sections were blocked with serum block for 15 minutes, then incubated overnight at 4°C with primary antibody diluted in PBS or TBS-T (1:1000 in PBS (β-catenin); 1:50 in TBS-T (BRAF)).
This product has been referenced in:
- Pestronk A et al. Immune myopathy with large histiocyte-related myofiber necrosis. Neurology 92:e1763-e1772 (2019). Read more (PubMed: 30894448) »
- Callahan SJ et al. Cancer modeling by Transgene Electroporation in Adult Zebrafish (TEAZ). Dis Model Mech 11:N/A (2018). Read more (PubMed: 30061297) »