Overview

  • Product name

  • Description

    Rabbit polyclonal to BRAF35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 98-315 of Human BRAF35 (Uniprot ID: Q9P0W2).

  • Positive control

    • 293T, Jurkat, Raji and A431 whole cell lysates; Human HBL435 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
  • Tissue specificity

    Ubiquitously expressed in adult tissues.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRAF25 antibody
    • BRAF35 antibody
    • BRCA2 associated factor 35 antibody
    • BRCA2-associated factor 35 antibody
    • High Mobility Group Protein 20B antibody
    • HM20B_HUMAN antibody
    • HMG box containing protein 20B antibody
    • HMG box-containing protein 20B antibody
    • HMG domain-containing protein 2 antibody
    • HMG domain-containing protein HMGX2 antibody
    • HMG20B antibody
    • SMARCE1-related protein antibody
    • SMARCE1r antibody
    • sox like antibody
    • Sox like transcriptional factor antibody
    • Sox-like transcriptional factor antibody
    • SOXL antibody
    • Structural DNA binding protein BRAF35 antibody
    • Structural DNA-binding protein BRAF35 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
    see all

Images

  • Anti-BRAF35 antibody (ab151286) at 1/2000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human HBL435 xenograft tissue labeling BRAF35 with ab151286 at 1/500 dilution.

References

This product has been referenced in:

  • Liu S  et al. Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. Cancer Med 6:819-833 (2017). WB . Read more (PubMed: 28332309) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151286.
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