Overview

  • Product name

  • Description

    Rabbit polyclonal to BRAF35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human BRAF35 (internal sequence). The exact sequence is proprietary.
    Database link: Q9P0W2

  • Positive control

    • IP: HEK-293T whole cell lysate/extract. WB: HEK-293T whole cell lysate. ICC/IF: A375 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
  • Tissue specificity

    Ubiquitously expressed in adult tissues.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRAF25 antibody
    • BRAF35 antibody
    • BRCA2 associated factor 35 antibody
    • BRCA2-associated factor 35 antibody
    • High Mobility Group Protein 20B antibody
    • HM20B_HUMAN antibody
    • HMG box containing protein 20B antibody
    • HMG box-containing protein 20B antibody
    • HMG domain-containing protein 2 antibody
    • HMG domain-containing protein HMGX2 antibody
    • HMG20B antibody
    • SMARCE1-related protein antibody
    • SMARCE1r antibody
    • sox like antibody
    • Sox like transcriptional factor antibody
    • Sox-like transcriptional factor antibody
    • SOXL antibody
    • Structural DNA binding protein BRAF35 antibody
    • Structural DNA-binding protein BRAF35 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
    see all

Images

  • Anti-BRAF35 antibody (ab228577) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa



    12% SDS-PAGE

  • A375 (human malignant melanoma cell line) cells stained for BRAF35 (green) using ab228577 (1;500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Counterstain: Hoechst 33342 (blue).

  • BRAF35 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using 2 µg of ab228577. Western blot was performed from the immunoprecipitate using ab228577 at a 1:1000 dilution.

    Lane 1: HEK-293T whole cell extracts 40 μg (Input).

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab228577 IP in HEK-293T whole cell extract.

    10% SDS-PAGE

References

ab228577 has not yet been referenced specifically in any publications.

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