Product nameAnti-BRAF35 antibody
See all BRAF35 primary antibodies
DescriptionRabbit polyclonal to BRAF35
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment within Human BRAF35 (internal sequence). The exact sequence is proprietary.
Database link: Q9P0W2
- IP: HEK-293T whole cell lysate/extract. WB: HEK-293T whole cell lysate. ICC/IF: A375 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 36 kDa.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/1000.|
FunctionRequired for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
Tissue specificityUbiquitously expressed in adult tissues.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
Cellular localizationNucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
- Information by UniProt
- BRAF25 antibody
- BRAF35 antibody
- BRCA2 associated factor 35 antibody
Anti-BRAF35 antibody (ab228577) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
A375 (human malignant melanoma cell line) cells stained for BRAF35 (green) using ab228577 (1;500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Counterstain: Hoechst 33342 (blue).
BRAF35 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using 2 µg of ab228577. Western blot was performed from the immunoprecipitate using ab228577 at a 1:1000 dilution.
Lane 1: HEK-293T whole cell extracts 40 μg (Input).
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab228577 IP in HEK-293T whole cell extract.
ab228577 has not yet been referenced specifically in any publications.