Overview

  • Product name

  • Description

    Rabbit polyclonal to BRAF35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 25 and 75 of human BRAF35 (NP_006330.2).

  • Positive control

    • Whole cell lysate from HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
  • Tissue specificity

    Ubiquitously expressed in adult tissues.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRAF25 antibody
    • BRAF35 antibody
    • BRCA2 associated factor 35 antibody
    • BRCA2-associated factor 35 antibody
    • High Mobility Group Protein 20B antibody
    • HM20B_HUMAN antibody
    • HMG box containing protein 20B antibody
    • HMG box-containing protein 20B antibody
    • HMG domain-containing protein 2 antibody
    • HMG domain-containing protein HMGX2 antibody
    • HMG20B antibody
    • SMARCE1-related protein antibody
    • SMARCE1r antibody
    • sox like antibody
    • Sox like transcriptional factor antibody
    • Sox-like transcriptional factor antibody
    • SOXL antibody
    • Structural DNA binding protein BRAF35 antibody
    • Structural DNA-binding protein BRAF35 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
    see all

Images

  • All lanes : Anti-BRAF35 antibody (ab72302) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Predicted band size: 36 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Detection of Human BRAF35 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72302 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References

ab72302 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
20 µg
Specification
lymphoblastoid cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Jan 13 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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