Key features and details
- Rabbit polyclonal to BRAF35
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BRAF35 antibody
See all BRAF35 primary antibodies
DescriptionRabbit polyclonal to BRAF35
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 25 and 75 of human BRAF35 (NP_006330.2).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
Tissue specificityUbiquitously expressed in adult tissues.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
Cellular localizationNucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
- Information by UniProt
- BRAF25 antibody
- BRAF35 antibody
- BRCA2 associated factor 35 antibody
All lanes : Anti-BRAF35 antibody (ab72302) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Detection of Human BRAF35 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72302 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
ab72302 has not yet been referenced specifically in any publications.