Overview

  • Product name
    Anti-BRAF35 antibody [EPR9915]
    See all BRAF35 primary antibodies
  • Description
    Rabbit monoclonal [EPR9915] to BRAF35
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human BRAF35 (UniProt: Q9P0W2)

  • Positive control
    • Jurkat, A431 and HeLa cell lysates. A431 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9915
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
    • Tissue specificity
      Ubiquitously expressed in adult tissues.
    • Sequence similarities
      Contains 1 HMG box DNA-binding domain.
    • Cellular localization
      Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRAF25 antibody
      • BRAF35 antibody
      • BRCA2 associated factor 35 antibody
      • BRCA2-associated factor 35 antibody
      • High Mobility Group Protein 20B antibody
      • HM20B_HUMAN antibody
      • HMG box containing protein 20B antibody
      • HMG box-containing protein 20B antibody
      • HMG domain-containing protein 2 antibody
      • HMG domain-containing protein HMGX2 antibody
      • HMG20B antibody
      • SMARCE1-related protein antibody
      • SMARCE1r antibody
      • sox like antibody
      • Sox like transcriptional factor antibody
      • Sox-like transcriptional factor antibody
      • SOXL antibody
      • Structural DNA binding protein BRAF35 antibody
      • Structural DNA-binding protein BRAF35 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
      see all

    Images

    • All lanes : Anti-BRAF35 antibody [EPR9915] (ab167415) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody

      Predicted band size: 35 kDa

    • Immunofluorescent analysis of A431 cells labeling BRAF35 with ab167415 at 1/100 dilution.

    References

    ab167415 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab167415.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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