Product nameBranched Chain Amino Acid Assay Kit / BCAA Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
Sensitivity> 0.2 nmol/well
Assay time0h 40m
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.
The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).
The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
BCAA assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader
The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests BCAA Assay Buffer WM 1 x 25ml BCAA Enzyme Mix (lyophilized) Green 1 vial Leu Standard (1 µmol) Yellow 1 x 100µl WST Substrate Mix (lyophilized) Red 1 vial
RelevanceThe branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects.
BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).
Leucine Standard Curve performed according to the attached protocol.
ab83374 has been referenced in 7 publications.
- Li J et al. Metabolic Changes in Spontaneously Hypertensive Rat Hearts Precede Cardiac Dysfunction and Left Ventricular Hypertrophy. J Am Heart Assoc 8:e010926 (2019). PubMed: 30764689
- Okazaki F et al. Microbiome Alteration in Type 2 Diabetes Mellitus Model of Zebrafish. Sci Rep 9:867 (2019). PubMed: 30696861
- Yoneshiro T et al. BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature 572:614-619 (2019). PubMed: 31435015
- Quattrocelli M et al. Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming. JCI Insight 4:N/A (2019). PubMed: 31852847
- Tsai S et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab 28:922-934.e4 (2018). PubMed: 30174303
- Serralde-Zúñiga AE et al. Omental adipose tissue gene expression, gene variants, branched-chain amino acids, and their relationship with metabolic syndrome and insulin resistance in humans. Genes Nutr 9:431 (2014). PubMed: 25260659
- Amann M et al. Peripheral fatigue limits endurance exercise via a sensory feedback-mediated reduction in spinal motoneuronal output. J Appl Physiol (1985) 115:355-64 (2013). PubMed: 23722705