Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (ab83374)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.2 nmol/well
Overview
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Product name
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit
See all Branched chain amino acid kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Sensitivity
> 0.2 nmol/well -
Assay time
0h 40m -
Product overview
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.
The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).
The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
BCAA assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K564 Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit. K564-100 is the same size as the 100 test size of ab83374.
The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer IV 1 x 25ml BCAA Enzyme Mix 1 vial Developer Solution III 1 vial Leucine Standard 1 x 100µl -
Research areas
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Relevance
The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects. -
Alternative names
- BCAA
- Isoleucine
- Leucine
- Valine
Associated products
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Related Products
Images
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BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
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BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).
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Leucine Standard Curve performed according to the attached protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab83374 has been referenced in 21 publications.
- Rebelo AR & Homem CCF dMyc-dependent upregulation of CD98 amino acid transporters is required for Drosophila brain tumor growth. Cell Mol Life Sci 80:30 (2023). PubMed: 36609617
- Hefetz L et al. Maternal antagonism of Glp1 reverses the adverse outcomes of sleeve gastrectomy on mouse offspring. JCI Insight 7:N/A (2022). PubMed: 35393955
- Zhang F et al. Excessive branched-chain amino acid accumulation restricts mesenchymal stem cell-based therapy efficacy in myocardial infarction. Signal Transduct Target Ther 7:171 (2022). PubMed: 35654769
- Fu SK et al. Effect of Daily Oral Lactobacillus plantarum PS128 on Exercise Capacity Recovery after a Half-Marathon. Nutrients 13:N/A (2021). PubMed: 34836278
- Bayon-Vicente G et al. Global Proteomic Analysis Reveals High Light Intensity Adaptation Strategies and Polyhydroxyalkanoate Production in Rhodospirillum rubrum Cultivated With Acetate as Carbon Source. Front Microbiol 11:464 (2020). PubMed: 32269553