Question (65259) | Branched Chain Amino Acid Assay Kit (ab83374)

Go to datasheet (ab83374)

Question

Hallo,

My client have a some questions about Branched chain amino acid assay kit # ab83374

Does using this kit can be determined the sum of branched chain amino acids (Leu + Ile + Val) ? Whether only leucine (standard contains the same leucine)?



BR.

Answer

Thank you for your inquiry.

I am happy to confirm that this kit will measure all the Branched chain amino acids (BCAAs).

All BCAAs will be deaminated to produce NADH, which is what this kit will actually measure.
The Leucine in the standard will also be converted to NADH. Therefore it does not matter which Branched chain amino acid is used as standard, it will still be measured in form of NADH.
Hope this information has been useful for you. Please let me know if you have any other questions.

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