Overview

  • Product name

    Anti-BRAP antibody
  • Description

    Rabbit polyclonal to BRAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human BRAP aa 13-124.
    Database link: Q7Z569

  • Positive control

    • WB: K562 whole cell lysate; Rat brain lysate. IHC-P: Human small intestine and colon cancer tissue. ICC/IF: PC-3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 67 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.

Target

  • Relevance

    BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 3010002G07Rik antibody
    • BRAP2 antibody
    • BRCA1 associated protein antibody
    • EC 6.3.2. antibody
    • Galectin 2 binding protein antibody
    • IMP antibody
    • Impedes mitogenic signal propagation antibody
    • Renal carcinoma antigen NY REN 63 antibody
    • RING finger protein 52 antibody
    • RNF52 antibody
    • zgc:92894 antibody
    see all

Images

  • All lanes : Anti-BRAP antibody (ab237505) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 67 kDa

  • Paraffin-embedded human small intestine tissue stained for BRAP using ab237505 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human colon cancer tissue stained for BRAP using ab237505 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • PC-3 (Human prostate adenocarcinoma cell line) cells stained for BRAP (green) using ab237505 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. 

     

References

ab237505 has not yet been referenced specifically in any publications.

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