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    brca1-antibody-ms110-ab16780.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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Anti-BRCA1 antibody [MS110] (ab16780)

  • Datasheet
  • SDS
Reviews (14)Q&A (9)References (45)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
  • Flow Cytometry - Anti-BRCA1 antibody [MS110] (ab16780)

Key features and details

  • Mouse monoclonal [MS110] to BRCA1
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-BRCA1 antibody [MS110]
    See all BRCA1 primary antibodies
  • Description

    Mouse monoclonal [MS110] to BRCA1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human BRCA1.

  • Epitope

    Within the N-terminal 304 amino acids of BRCA1.
  • Positive control

    • IHC-P: Human breast carcinoma tissue. Human skin tissue. ICC/IF: MCF7 and A431 cells. Human ovarian tumor cells. Human colon cancer cells. Flow cytometry: MCF7 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Please note that this antibody is not suitable for WB.
    Despite positive publications and Abreviews we have mixed feedback on this antibody in WB and we do not guarantee ab16780 for WB.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    MS110
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Anti-BRCA1 antibody [MS110] - BSA and Azide free (ab264518)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human BRCA1 protein (ab82204)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (4)
Use at an assay dependent concentration.
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
    • Tissue specificity

      Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Defects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
      Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
      Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
    • Sequence similarities

      Contains 2 BRCT domains.
      Contains 1 RING-type zinc finger.
    • Domain

      The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
      The RING-type zinc finger domain interacts with BAP1.
    • Post-translational
      modifications

      Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
      Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
    • Cellular localization

      Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.
    • Target information above from: UniProt accession P38398 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 672 Human
      • Omim: 113705 Human
      • SwissProt: P38398 Human
      • Unigene: 194143 Human
      • Alternative names

        • BRCA 1 antibody
        • BRCA1 antibody
        • BRCA1 DNA repair associated antibody
        • BRCA1/BRCA2 containing complex subunit 1 antibody
        • BRCA1/BRCA2-containing complex, subunit 1 antibody
        • BRCA1_HUMAN antibody
        • BRCAI antibody
        • BRCC 1 antibody
        • BRCC1 antibody
        • Breast and ovarian cancer susceptibility protein 1 antibody
        • Breast Cancer 1 antibody
        • Breast Cancer 1 Early Onset antibody
        • Breast cancer type 1 susceptibility protein antibody
        • BROVCA1 antibody
        • FANCS antibody
        • IRIS antibody
        • PNCA4 antibody
        • PPP1R53 antibody
        • Protein phosphatase 1 regulatory subunit 53 antibody
        • PSCP antibody
        • RING finger protein 53 antibody
        • RNF53 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)

        IHC image of ab16780 staining in normal human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16780, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

        *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
        IHC image of ab16780 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16780, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS110] (ab16780)This image is courtesy of an Anonymous Abreview.

        ab16780 staining BRCA1 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 2% BSA for 1 hour at 22°C. The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C. A biotin-conjugated goat anti-mouse polyclonal was used as the secondary antibody, diluted 1/800. Antibody was detected by DAB staining.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)

        ICC/IF image of ab16780 stained MCF7cells. The cells were 4% PFA fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16780, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

      • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)Image is courtesy of an anonymous Abreview

        ab16780 staining BRAC1 in human ovarian tumor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Acetone:Methanol and blocked with a protein block, serum-free for 1 hour at 18°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Rabbit anti-mouse IgG (H+L) polyclonal (1/400) was used as the secondary antibody.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)Image is courtesy of an anonymous Abreview

        ab16780 staining BRAC1 in human colon cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 30 minutes at 4°C. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)
        Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS110] (ab16780)This image is courtesy of an Abreview submitted by Dr Alejandro Vazquez-Martin
        ab16780 staining BRCA1 in human A431 epidermoid cancer cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (1/50 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-mouse polyclonal (1/50) was used as the secondary.

        See Abreview

      • Flow Cytometry - Anti-BRCA1 antibody [MS110] (ab16780)
        Flow Cytometry - Anti-BRCA1 antibody [MS110] (ab16780)

        Overlay histogram showing MCF7 cells stained with ab16780 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16780, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (45)

      Publishing research using ab16780? Please let us know so that we can cite the reference in this datasheet.

      ab16780 has been referenced in 45 publications.

      • Sang M  et al. Effect of AR antagonist combined with PARP1 inhibitor on sporadic triple-negative breast cancer bearing AR expression and methylation-mediated BRCA1 dysfunction. Biomed Pharmacother 111:169-177 (2019). PubMed: 30580238
      • Tan X  et al. Dynamically decreased miR-671-5p expression is associated with oncogenic transformation and radiochemoresistance in breast cancer. Breast Cancer Res 21:89 (2019). PubMed: 31391072
      • Guo Y  et al. Filamin A inhibits tumor progression through regulating BRCA1 expression in human breast cancer. Oncol Lett 16:6261-6266 (2018). PubMed: 30405761
      • Luo Y  et al. ß-catenin nuclear translocation induced by HIF-1a overexpression leads to the radioresistance of prostate cancer. Int J Oncol 52:1827-1840 (2018). PubMed: 29658569
      • Kim S  et al. Regulating BRCA1 protein stability by cathepsin S-mediated ubiquitin degradation. Cell Death Differ N/A:N/A (2018). PubMed: 30006610
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 23 Abreviews or Q&A

      Question

      Lisates were prepaired in ice using RIPA buffer, from human lung cancer cell lines: SKLMS, CALU-1, A549; The dilution of primary antibody was 1:500; Secondary antibody was used anti-mouse from LSbio, lysates were heated during 1 minute. Unfortunately I still don't have an order number from secretary.
      I've attached the most representative image on this e-mail.
      Thank you for your time,
      Sincerely,

      Read More

      Abcam community

      Verified customer

      Asked on Sep 28 2012

      Answer

      Thank you for your email.

      As observed in image the problem is more with the protocol rather than antibody. Please try troubleshooting as follows;

      - Heat lysates with sample buffer for 5-10 minutes at 100C
      - Milk, blocking agent is not recommend for phospho-specific antibody e.g. ab47276; please try 5% BSA
      - ab16780 is mouse monoclonal antibody and ab47276 is rabbit polyclonal. Anti mouse antibody would not be working with ab47276.
      - Please try 4X5 minutes and 1, 10 minutes wash in TBST buffer.
      - It seems the lysates are loaded at high amount. If lysates concentration is correct then I would recommend trying 10ug lysates.

      I hope the suggestions will help to improve results.

      Read More

      Abcam Scientific Support

      Answered on Sep 28 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BRCA1 antibody [MS110]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (HT29 tumor taken out Balb/C nude mice)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Ph6
      Permeabilization
      No
      Specification
      HT29 tumor taken out Balb/C nude mice
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 20 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-BRCA1 antibody [MS110]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Vascular smooth muscle cell)
      Permeabilization
      Yes - NP40
      Specification
      Vascular smooth muscle cell
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 26 2018

      Western blot abreview for Anti-BRCA1 antibody [MS110]

      Below Average
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Endothelial)
      Gel Running Conditions
      Reduced Denaturing (7%)
      Loading amount
      50 µg
      Specification
      Endothelial
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 05 2017

      Immunoprecipitation abreview for Anti-BRCA1 antibody [MS110]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Immuno-precipitation step
      Protein G
      Sample
      Human Cell lysate - whole cell (Hela cells)
      Specification
      Hela cells
      Total protein in input
      2000 µg
      Read More

      Dr. Remi Buisson

      Verified customer

      Submitted Jan 05 2015

      Western blot abreview for Anti-BRCA1 antibody [MS110]

      Average
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Sample
      Human Cell lysate - whole cell (UWB1.289 cells)
      Specification
      UWB1.289 cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. Remi Buisson

      Verified customer

      Submitted Nov 17 2014

      Western blot abreview for Anti-BRCA1 antibody [MS110]

      Below Average
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Ovarian Tumor)
      Gel Running Conditions
      Reduced Denaturing (3-8%)
      Loading amount
      30 µg
      Specification
      Ovarian Tumor
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 05 2014

      Immunocytochemistry/ Immunofluorescence abreview for Anti-BRCA1 antibody [MS110]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 18°C
      Sample
      Human Cell (Ovarian Tumor)
      Specification
      Ovarian Tumor
      Permeabilization
      Yes - Acetone:Methanol
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 02 2014

      Western blot abreview for Anti-BRCA1 antibody [MS110]

      Average
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (6%)
      Sample
      Human Cell lysate - whole cell (U2OS osteosarcoma)
      Specification
      U2OS osteosarcoma
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 25 2014

      Western blot abreview for Anti-BRCA1 antibody [MS110]

      Good
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (6%)
      Sample
      Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
      Specification
      Mouse breast cancer cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 15 2013

      1-10 of 23 Abreviews or Q&A

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