Western blot abreview for Anti-BRCA1 (phospho S1524) antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T Cells)
Loading amount
30 µg
Specification
293T Cells
Treatment
10uM Bleomycin for 30 min.
Gel Running Conditions
Reduced Denaturing (6% Acrylamide Gel)
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 680 anti-Rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 220 kDa

Abcam user community

Verified customer

Submitted Nov 18 2009

Sign up