Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells (Cervical Cancer))
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa Cells (Cervical Cancer)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
45 minute(s) · Temperature: 37°C · Diluent: PBS + 3% BSA
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Jackson Labs FITC Goat Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Abcam response
Thank you very much for your valuable feedback. We are pleased that this antibody worked well for you in ICC which has not been tested before.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Aug 04 2015