Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma and Melanoma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Glioblastoma and Melanoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
N/A
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 245 kDa Non-specific: ~280, ~330 kDa
Positive control
Non-treated cell
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Apr 22 2019