Application
Western blot
Sample
Human Cell lysate - whole cell (HT29 cell lysate)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount
50 µg
Specification
HT29 cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal west Femto
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 384 kDa Non-specific: 55, 60, 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 20 2020