Application
Western blot
Sample
Mouse Cell lysate - whole cell (CT26 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Tris-Bis gel)
Loading amount
40 µg
Specification
CT26 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Immpress Goat anti-rabbit IGG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
FemtoECL
Exposure
12 minute(s) and 0 second(s)
Additional data
Additional Notes
antibody not suitable for mice sample.
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product has not yet been tested in this species, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 17 2022