Western blot abreview for Anti-BRCA2 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
293T

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TTBS + 1% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 350 kDa Non-specific: 250 kDa

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Submitted Nov 02 2016

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