Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-BRCC36 antibody [EPR4366] (ab108411)

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Overview

  • Product name

    Anti-BRCC36 antibody [EPR4366]
    See all BRCC36 primary antibodies

  • Description

    Rabbit monoclonal [EPR4366] to BRCC36

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BRCC36 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, MCF7, SKBR-3, HAP1 and 293T cell lysates Flow Cyt: permeabilized HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).

    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.

    • Involvement in disease

      A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.

    • Sequence similarities

      Belongs to the peptidase M67A family. BRCC36 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.

    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BRCA1-A complex subunit BRCC36 antibody
      • BRCA1/BRCA2 containing complex subunit 3 antibody
      • BRCA1/BRCA2 containing complex subunit 36 antibody
      • BRCA1/BRCA2-containing complex subunit 3 antibody
      • BRCA1/BRCA2-containing complex subunit 36 antibody
      • Brcc3 antibody
      • BRCC3_HUMAN antibody
      • BRCC36 antibody
      • BRISC complex subunit BRCC36 antibody
      • C6.1A antibody
      • Chromosome X open reading frame 53 antibody
      • CXorf53 antibody
      • Lys-63-specific deubiquitinase BRCC36 antibody
      • OTTHUMP00000061450 antibody
      see all

    Images

    • Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
      Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: Empty Lane
      Lane 3: BRCC3 knockout HAP1 whole cell lysate (40 µg)
      Lane 4: MCF7 whole cell lysate (40 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108411 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108411 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE.  Ab108411 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
      Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
      All lanes : Anti-BRCC36 antibody [EPR4366] (ab108411) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)
      Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)
      Flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).

    References

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