Anti-BRCC36 antibody [EPR4366] (ab108411)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-BRCC36 antibody [EPR4366]
See all BRCC36 primary antibodies -
DescriptionRabbit monoclonal [EPR4366] to BRCC36
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human BRCC36 aa 200-300. The exact sequence is proprietary.
-
Positive control
- WB: HeLa, MCF7, SKBR-3, HAP1 and 293T cell lysates Flow Cyt: permeabilized HeLa cells
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR4366
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
FunctionMetalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
-
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.
-
Involvement in diseaseA chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
-
Sequence similaritiesBelongs to the peptidase M67A family. BRCC36 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
Cellular localizationNucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
- Information by UniProt
-
Database links
- Entrez Gene: 79184 Human
- Entrez Gene: 210766 Mouse
- Entrez Gene: 316794 Rat
- Omim: 300617 Human
- SwissProt: P46736 Human
- SwissProt: P46737 Mouse
- SwissProt: B2RYM5 Rat
- Unigene: 558537 Human
see all -
Alternative names
- BRCA1-A complex subunit BRCC36 antibody
- BRCA1/BRCA2 containing complex subunit 3 antibody
- BRCA1/BRCA2 containing complex subunit 36 antibody
see all
Images
-
![Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)](https://www.abcam.com/ps/products/108/ab108411/Images/ab108411-280991-ab108411KOWB.jpg)
Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: Empty Lane
Lane 3: BRCC3 knockout HAP1 whole cell lysate (40 µg)
Lane 4: MCF7 whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108411 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108411 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab108411 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)](https://www.abcam.com/ps/products/108/ab108411/Images/BRCC36-Primary-antibodies-ab108411-1.jpg)
Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)All lanes : Anti-BRCC36 antibody [EPR4366] (ab108411) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa -
![Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)](https://www.abcam.com/ps/products/108/ab108411/Images/BRCC36-Primary-antibodies-ab108411-2.jpg)
Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)Flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
References
ab108411 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"