Overview

  • Product name

  • Description

    Rabbit polyclonal to BRCC45/BRE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human BRCC45/BRE aa 9-338.
    Database link: NP_004890

  • Positive control

    • HepG2, HeLa and NIH-3T3 whole cell lysates; 293T, A431, H1299, HeLaS3, MOLT4 and Raji cell lysates ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as BRCC45

     

Properties

Applications

Our Abpromise guarantee covers the use of ab95984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • Tissue specificity

    Expressed in all cell lines examined. Highly expressed in placenta.
  • Sequence similarities

    Belongs to the BRE family.
  • Domain

    Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • Cellular localization

    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Images

  • All lanes : Anti-BRCC45/BRE antibody (ab95984) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : BRCC45/BRE Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 47 kDa

  • All lanes : Anti-BRCC45/BRE antibody (ab95984) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of BRCC45/BRE in paraformaldehyde fixed HeLa, using ab95984 at 1/200 dilution. Lower image merged with DNA probe.

  • Anti-BRCC45/BRE antibody (ab95984) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    12% SDS PAGE

References

ab95984 has not yet been referenced specifically in any publications.

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