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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Submit a review Submit a question References (2)
Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11858] to BRCC45/BRE
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BRCC45/BRE antibody [EPR11858]
    See all BRCC45/BRE primary antibodies

  • Description

    Rabbit monoclonal [EPR11858] to BRCC45/BRE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
    Database link: Q9NXR7

  • Positive control

    • WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.

  • Tissue specificity

    Expressed in all cell lines examined. Highly expressed in placenta.

  • Sequence similarities

    Belongs to the BRE family.

  • Domain

    Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.

  • Cellular localization

    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Images

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BRCC45/BRE knockout HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

References (2)

Publishing research using ab177960? Please let us know so that we can cite the reference in this datasheet.

ab177960 has been referenced in 2 publications.

  • Jin F  et al. BRE Promotes Esophageal Squamous Cell Carcinoma Growth by Activating AKT Signaling. Front Oncol 10:1407 (2020). PubMed: 32850455
  • Ramos H  et al. Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Antioxidants (Basel) 9:N/A (2020). PubMed: 32927585

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