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  1. Link

    brcc45bre-antibody-epr11858-ab177960.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

  • Datasheet
  • SDS
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Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
  • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11858] to BRCC45/BRE
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human BRCC45/BRE protein (ab160555)
Primary
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Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
Knockout
Product image
Human BRE (BRCC45) knockout HeLa cell line (ab264928)

View more associated products

Overview

  • Product name

    Anti-BRCC45/BRE antibody [EPR11858]
    See all BRCC45/BRE primary antibodies
  • Description

    Rabbit monoclonal [EPR11858] to BRCC45/BRE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
    Database link: Q9NXR7

  • Positive control

    • WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11858
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway

Associated products

  • Alternative Versions

    • Anti-BRCC45/BRE antibody [EPR11858] - BSA and Azide free (ab250030)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BRE (BRCC45) knockout HeLa cell line (ab264928)
  • KO cell lysates

    • Human BRE (BRCC45) knockout HeLa cell lysate (ab257861)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human BRCC45/BRE protein (ab160555)
  • Related Products

    • Recombinant Human BRCC45/BRE protein (ab160555)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • Tissue specificity

    Expressed in all cell lines examined. Highly expressed in placenta.
  • Sequence similarities

    Belongs to the BRE family.
  • Domain

    Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • Cellular localization

    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Target information above from: UniProt accession Q9NXR7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9577 Human
    • Entrez Gene: 107976 Mouse
    • Entrez Gene: 362704 Rat
    • Omim: 610497 Human
    • SwissProt: Q9NXR7 Human
    • SwissProt: Q8K3W0 Mouse
    • SwissProt: Q6P7Q1 Rat
    • Unigene: 258314 Human
    • Unigene: 482126 Mouse
    • Unigene: 224501 Rat
    see all
  • Alternative names

    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Images

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BRCC45/BRE knockout HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab177960? Please let us know so that we can cite the reference in this datasheet.

ab177960 has been referenced in 2 publications.

  • Jin F  et al. BRE Promotes Esophageal Squamous Cell Carcinoma Growth by Activating AKT Signaling. Front Oncol 10:1407 (2020). PubMed: 32850455
  • Ramos H  et al. Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Antioxidants (Basel) 9:N/A (2020). PubMed: 32927585

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