Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11858] to BRCC45/BRE
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRCC45/BRE antibody [EPR11858]
    See all BRCC45/BRE primary antibodies

  • Description

    Rabbit monoclonal [EPR11858] to BRCC45/BRE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
    Database link: Q9NXR7

  • Positive control

    • WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.

  • General notes

     This product was previously labelled as BRCC45

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab177960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.

    • Tissue specificity

      Expressed in all cell lines examined. Highly expressed in placenta.

    • Sequence similarities

      Belongs to the BRE family.

    • Domain

      Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.

    • Cellular localization

      Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 6030405P19Rik antibody
      • AI429776 antibody
      • B830038C02Rik antibody
      • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
      • brain and reproductive organ expressed protein antibody
      • Brain and reproductive organ-expressed protein antibody
      • BRCA1 A complex subunit BRE antibody
      • BRCA1-A complex subunit BRE antibody
      • BRCA1/BRCA2 containing complex subunit 4 antibody
      • BRCA1/BRCA2 containing complex subunit 45 antibody
      • BRCA1/BRCA2-containing complex subunit 45 antibody
      • BRCC4 antibody
      • BRCC45 antibody
      • bre antibody
      • BRE_HUMAN antibody
      see all

    Images

    • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BRCC45/BRE knockout HeLa cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 44 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.

       ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

      Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

      Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : Human placenta lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

      Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
      Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    References (0)

    Publishing research using ab177960? Please let us know so that we can cite the reference in this datasheet.

    ab177960 has not yet been referenced specifically in any publications.

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