Product nameAnti-BRD1/BRL antibody
See all BRD1/BRL primary antibodies
DescriptionRabbit polyclonal to BRD1/BRL
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BRD1/BRL aa 750-800. The exact sequence is proprietary. NP_055392.1
Database link: O95696
- WB: HeLa nuclear extract. IP: HeLa nuclear extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 120 kDa.|
FunctionComponent of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
Tissue specificityHighly expressed in testis.
Sequence similaritiesContains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
- Information by UniProt
- BR140-like protein antibody
- BRD1 antibody
- BRD1_HUMAN antibody
All lanes : Anti-BRD1/BRL antibody (ab264293) at 0.1 µg/ml
Lane 1 : HeLa nuclear extract at 50 µg
Lane 2 : HeLa nuclear extract at 15 µg
Lane 3 : HeLa nuclear extract at 5 µg
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 120 kDa
Exposure time: 30 seconds
BRD1/BRL was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract with ab264293 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264293 at 0.4 µg/ml.
Lane 1 &2: ab264293 IP in HeLa nuclear extract.
Lane 3: Control IgG.
Exposure time: 30 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264293 has not yet been referenced specifically in any publications.