Anti-BRD1/BRL antibody (ab71877)
Key features and details
- Rabbit polyclonal to BRD1/BRL + Peregrin/BRPF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-BRD1/BRL antibody -
Description
Rabbit polyclonal to BRD1/BRL + Peregrin/BRPF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) | |
ICC/IF |
Notes |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Cellular localization
BRD1/BRL: Nucleus. Peregrin/BRPF1: Nucleus. Cytoplasm. Localization to the nucleus depends on KAT6A, ING5 and MEAF6. -
Database links
- Entrez Gene: 417731 Chicken
- Entrez Gene: 427590 Chicken
- Entrez Gene: 23774 Human
- Entrez Gene: 7862 Human
- Entrez Gene: 223770 Mouse
- Entrez Gene: 78783 Mouse
- Omim: 602410 Human
- Omim: 604589 Human
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Images
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All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 :Raji whole cell lysate (ab30124) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
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All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : Lysates prepared from Mouse testis tissue.
Lane 2 : Lysates prepared from Mouse Lung tissue.
Lane 3 : Lysates prepared from Mouse Kidney tissue.
Lane 4 : Lysates prepared from GC-1 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 7 minutesGC-1 - mouse spermatogonia cell line
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab71877 has been referenced in 2 publications.
- Wu Q et al. A chemical toolbox for the study of bromodomains and epigenetic signaling. Nat Commun 10:1915 (2019). PubMed: 31015424
- Kang H et al. Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila. Genes Dev 31:1988-2002 (2017). PubMed: 29070704