Overview

  • Product name
  • Description
    Rabbit polyclonal to BRD1/BRL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human BRD1/BRL aa 150-250 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab71876)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: TE 671 Raji
  • General notes

     This product was previously labelled as BRD1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab71877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
    • Tissue specificity
      Highly expressed in testis.
    • Sequence similarities
      Contains 1 bromo domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 PWWP domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BR140-like protein antibody
      • BRD1 antibody
      • BRD1_HUMAN antibody
      • BRL antibody
      • Bromodomain and PHD finger-containing protein 2 antibody
      • Bromodomain containing 1 antibody
      • Bromodomain-containing protein 1 antibody
      • BRPF1 antibody
      • BRPF2 antibody
      • DKFZp686F0325 antibody
      see all

    Images

    • All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml

      Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 2 : Raji whole cell lysate (ab30124) at 10 µg

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 137 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
    • ab71877 staining BRD1/BRL in mouse testis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C was performed. The primary antibody was diluted 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

      The image indicated a specific nuclear staining and very low background. Arrows indicate some of the positive cells.

      See Abreview

    • All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml

      Lane 1 : Lysates prepared from Mouse testis tissue.
      Lane 2 : Lysates prepared from Mouse Lung tissue.
      Lane 3 : Lysates prepared from Mouse Kidney tissue.
      Lane 4 : Lysates prepared from GC-1 cell line.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 137 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?
      Additional bands at: 34 kDa, 92 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 7 minutes


      GC-1 - mouse spermatogonia cell line

      See Abreview

    References

    This product has been referenced in:
    • Kang H  et al. Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila. Genes Dev 31:1988-2002 (2017). Read more (PubMed: 29070704) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (testis)
    Specification
    testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 14 2010

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Ms Testis, Ms Lung, Ms Kidney, GC-1)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Ms Testis, Ms Lung, Ms Kidney, GC-1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 12 2010

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