Overview

  • Product name
  • Description
    Goat polyclonal to BRD2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Bromodomain Containing 2 (BRD 2) between the putative nuclear localization signal and the ET domain.

  • Positive control
    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/40000. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 22215678

Target

Images

  • Sample: Hela cell total RIPA extract (10µg)

    Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000)

    CIP: Calf Intestinal Phosphatase

    Detection: ECL exposed for 1 minute.

     

    Sample: Hela cell total RIPA extract (10µg) Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000) CIP: Calf Intestinal Phosphatase Detection: ECL exposed for 1 minute.

References

This product has been referenced in:
  • Sun Y  et al. BET protein inhibition mitigates acute myocardial infarction damage in rats via the TLR4/TRAF6/NF-?B pathway. Exp Ther Med 10:2319-2324 (2015). WB . Read more (PubMed: 26668635) »
  • Dhar S  et al. Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis. J Biol Chem 287:6387-405 (2012). ICC/IF ; Rat . Read more (PubMed: 22215678) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount
15 µg
Specification
embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 22 2013

Question

Dear Josephine,



Thank you for your reply.



This antibody is supposed to bind with both the phosphorylated and unphosphorylated brd2 protein, isn`t it?

The recommended dilution of ab3718 for western blot is 1/10000 - 1/40000, and what about ab113117?

Best regards

Caam Wong







发件人: technical@abcam.com [mailto:technical@abcam.com]
发送时间: 2012年5月2日 13:42
收件人: 11111220061@fudan.edu.cn
主题: Reply from Abcam to your enquiry regarding ab3718 [CCE3731884]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Caam

Thank you for contacting us.

The target protein got a number of phosphorserine and phosphothreonine residues which can increase the molecular weight of the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews



Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3731884


Best regards,
Josephine

Josephine Au, PhD
Scientific Support Supervisor
Abcam (Hong Kong) Ltd.
www.abcam.com


Your original inquiry to Abcam:


Name: Caam Wong

Product code: 3718



Inquiry: Dear Sir/Madam, I am interested in your brd2 antibody. I am wondering that the MW of brd2 is supposed to be around 90kd, why it is 110kd on your website? Thank you!




Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3731884]













Discover more at abcam.com

Read More
Answer

Thank you for contacting us.

Yes, ab3718 can recognise both the phosphorylated and unphosphorylated BRD2.

For ab113117, we recommend to use 1ug/ml (1:1000) in WB.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

The target protein got a number of phosphorserine and phosphothreonine residues which can increase the molecular weight of the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your enquiry. I have requested that the lab performs an alignment of the immunogen sequences with the rat homologs. Details of the degree of similarity is below: BRD2(rat) - GeneID: 294276 (NP_997660) The peptide immunogen was 14aa. There are two mismatches (conserved substitutions) between the rat and human at this site. The other 12 residues are identical. Probability of reactivity with rat is likely in the range of 50 to 70%. Tbp (rat) - GeneID: 117526 (NP_001004198) The immunogen used to raise rabbit polyclonal to TBP (ab24342) was found to have 100% sequence identity to rat TBP. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up