Product nameAnti-BRD2 antibody [EPR7642]
See all BRD2 primary antibodies
DescriptionRabbit monoclonal [EPR7642] to BRD2
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human BRD2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P25440
- WB: Jurkat, MOLT4, NCCIT and HeLa whole cell lysate (ab150035). ICC/IF: HeLa and wild-type HAP1 cells. IHC-P: Human testis tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 488) (ab197865)
- Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 647) (ab197866)
- Anti-BRD2 antibody [EPR7642] (HRP) (ab198536)
- Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 568) (ab211988)
- Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 555) (ab213347)
- Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 594) (ab215507)
- Anti-BRD2 antibody [EPR7642] - BSA and Azide free (ab222393)
Our Abpromise guarantee covers the use of ab139690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 88 kDa.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 µg/ml.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay play a role in spermatogenesis or folliculogenesis.
Sequence similaritiesContains 2 bromo domains.
Contains 1 ET domain.
DomainOne bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
- Information by UniProt
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRD2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab139690observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab139690 was shown to specifically react with BRD2 when BRD2 knockout samples were used. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. ab139690 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab139690 staining Brd2 in wild-type HAP1 cells (top panel) and BRD2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab139690 at 0.5μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Overlay histogram showing HeLa cells stained with ab139690 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139690, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-BRD2 antibody [EPR7642] (ab139690) at 1/1000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : NCCIT cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa
Immunofluorescence analysis of HeLa cells labeling BRD2, with ab139690 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD2 with ab139690 at 1/250 dilution.
This product has been referenced in:
- Zhang B et al. BRCA1 deficiency sensitizes breast cancer cells to bromodomain and extra-terminal domain (BET) inhibition. Oncogene N/A:N/A (2018). Read more (PubMed: 30042414) »
- Chan KH et al. Impact of Target Warhead and Linkage Vector on Inducing Protein Degradation: Comparison of Bromodomain and Extra-Terminal (BET) Degraders Derived from Triazolodiazepine (JQ1) and Tetrahydroquinoline (I-BET726) BET Inhibitor Scaffolds. J Med Chem 61:504-513 (2018). Read more (PubMed: 28595007) »