Overview

  • Product name

    Anti-BRD2 antibody [EPR7642] - BSA and Azide free
    See all BRD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7642] to BRD2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, ICC/IF, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P25440

  • Positive control

    • WB: Jurkat, MOLT4, NCCIT and HeLa cell lysates. ICC/IF: HeLa and wild-type HAP1 cells. IHC-P: Human testis tissue.
  • General notes

    Ab222393 is the carrier-free version of ab139690. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May play a role in spermatogenesis or folliculogenesis.
    • Sequence similarities

      Contains 2 bromo domains.
      Contains 1 ET domain.
    • Domain

      One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • BRD 2 antibody
      • Brd2 antibody
      • BRD2_HUMAN antibody
      • Bromodomain containing 2 antibody
      • Bromodomain-containing protein 2 antibody
      • D6S113E antibody
      • DKFZp686N0336 antibody
      • Female sterile homeotic related gene 1 antibody
      • Female sterile homeotic related gene 1, mouse, homolog of antibody
      • FLJ31942 antibody
      • FSH antibody
      • FSRG1 antibody
      • KIAA9001 antibody
      • NAT antibody
      • O27.1.1 antibody
      • Really interesting new gene 3 protein antibody
      • RING3 antibody
      • RING3 PROTEIN antibody
      • RNF3 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD2 with ab139690 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab139690 staining Brd2 in wild-type HAP1 cells (top panel) and BRD2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab139690 at 0.5μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).

    • Chromatin was prepared from HT-29 cells according to the Abcam X-ChIP protocol*. Cells were fixed with formaldehyde for 10 minutes.
      The ChIP was performed with 25 µg of chromatin, 5 µg of ab139690 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
      Primers and probes are located in the first kb of the transcribed region.
      *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).
    • Overlay histogram showing HeLa cells stained with ab139690 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139690, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).

    • Chromatin was prepared from LNCaP cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with formaldehyde for 10 minutes.
      The ChIP was performed with 25 µg of chromatin, 5 µg of ab139690 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
      Primers and probes are located in the first kb of the transcribed region.
      *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).

    • Immunofluorescence analysis of HeLa cells labeling BRD2, with ab139690 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139690).

    References

    ab222393 has not yet been referenced specifically in any publications.

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