Overview

  • Product name

    Anti-BRD2 antibody [EPR7642] (HRP)
    See all BRD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7642] to BRD2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BRD2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P25440

  • Positive control

    • WB: MOLT4, Jurkat, NCCIT and HeLa whole cell lysates.
  • General notes

    Alternative versions available:
    Anti-BRD2 antibody [EPR7642] (ab139690) Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).

Target

  • Function

    May play a role in spermatogenesis or folliculogenesis.
  • Sequence similarities

    Contains 2 bromo domains.
    Contains 1 ET domain.
  • Domain

    One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRD 2 antibody
    • Brd2 antibody
    • BRD2_HUMAN antibody
    • Bromodomain containing 2 antibody
    • Bromodomain-containing protein 2 antibody
    • D6S113E antibody
    • DKFZp686N0336 antibody
    • Female sterile homeotic related gene 1 antibody
    • Female sterile homeotic related gene 1, mouse, homolog of antibody
    • FLJ31942 antibody
    • FSH antibody
    • FSRG1 antibody
    • KIAA9001 antibody
    • NAT antibody
    • O27.1.1 antibody
    • Really interesting new gene 3 protein antibody
    • RING3 antibody
    • RING3 PROTEIN antibody
    • RNF3 antibody
    see all

Images

  • All lanes : Anti-BRD2 antibody [EPR7642] (HRP) (ab198536) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : BRD2 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    ab198536 was shown to specifically react with BRD2 in wild-type HAP1 cells as signal was lost in BRD2 knockout cells. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. Ab198536 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.

  • All lanes : Anti-BRD2 antibody [EPR7642] (HRP) (ab198536) at 1/5000 dilution

    Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NCCIT (Human pluripotent embryonal teratocarcinoma) Whole Cell Lysate
    Lane 4 : HeLa whole cell lysate (ab150035)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198536 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab198536 has not yet been referenced specifically in any publications.

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