Recombinant Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22114] to BRD2 (phospho S37)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BRD2 (phospho S37) antibody [EPR22114]
See all BRD2 primary antibodies -
Description
Rabbit monoclonal [EPR22114] to BRD2 (phospho S37) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Dot Blot: BRD2 (phospho S37) peptide (aa31-41) and BRD2 (phospho S37) peptide (aa34-44). WB: Untreated HEK-293 whole cell lysate and HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22114 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
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Notes |
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Dot blot
1/1000. |
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). |
Target
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Function
May play a role in spermatogenesis or folliculogenesis. -
Sequence similarities
Contains 2 bromo domains.
Contains 1 ET domain. -
Domain
One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6046 Human
- Omim: 601540 Human
- SwissProt: P25440 Human
- Unigene: 75243 Human
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Alternative names
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
see all
Images
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All lanes : Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255) at 1/5000 dilution
Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate
Lane 3 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking/diluting buffer: 5% NFDM/TBST.
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Dot blot analysis of BRD2 (phospho S37) labeled with ab223255 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/1000,000 dilution.
Lane 1: BRD2 (phospho S37) peptide (aa 31-41).
Lane 2: BRD2 (phospho S37) peptide (aa 34-44).
Lane 3: BRD2 non-phospho peptide (aa 31-41).
Exposure time: 10 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab223255 has not yet been referenced specifically in any publications.