Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22114] to BRD2 (phospho S37)
- Suitable for: Dot blot, WB
- Reacts with: Human
Product nameAnti-BRD2 (phospho S37) antibody [EPR22114]
See all BRD2 primary antibodies
DescriptionRabbit monoclonal [EPR22114] to BRD2 (phospho S37)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BRD2 aa 1-100 (phospho S37). The exact sequence is proprietary.
Database link: P25440
- Dot Blot: BRD2 (phospho S37) peptide (aa31-41) and BRD2 (phospho S37) peptide (aa34-44). WB: Untreated HEK-293 whole cell lysate and HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab223255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).|
FunctionMay play a role in spermatogenesis or folliculogenesis.
Sequence similaritiesContains 2 bromo domains.
Contains 1 ET domain.
DomainOne bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
- Information by UniProt
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
All lanes : Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255) at 1/5000 dilution
Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate
Lane 3 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 92 seconds
Blocking/diluting buffer: 5% NFDM/TBST.
Dot blot analysis of BRD2 (phospho S37) labeled with ab223255 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/1000,000 dilution.
Lane 1: BRD2 (phospho S37) peptide (aa 31-41).
Lane 2: BRD2 (phospho S37) peptide (aa 34-44).
Lane 3: BRD2 non-phospho peptide (aa 31-41).
Exposure time: 10 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223255 has not yet been referenced specifically in any publications.