Product nameAnti-BRD2 (phospho S37) antibody [EPR22114]
See all BRD2 primary antibodies
DescriptionRabbit monoclonal [EPR22114] to BRD2 (phospho S37)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BRD2 aa 1-100 (phospho S37). The exact sequence is proprietary.
Database link: P25440
- Dot Blot: BRD2 (phospho S37) peptide (aa31-41) and BRD2 (phospho S37) peptide (aa34-44). WB: Untreated HEK-293 whole cell lysate and HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab223255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).|
FunctionMay play a role in spermatogenesis or folliculogenesis.
Sequence similaritiesContains 2 bromo domains.
Contains 1 ET domain.
DomainOne bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
- Information by UniProt
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
All lanes : Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255) at 1/5000 dilution
Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate
Lane 3 : HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 92 seconds
Blocking/diluting buffer: 5% NFDM/TBST.
Dot blot analysis of BRD2 (phospho S37) labeled with ab223255 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/1000,000 dilution.
Lane 1: BRD2 (phospho S37) peptide (aa 31-41).
Lane 2: BRD2 (phospho S37) peptide (aa 34-44).
Lane 3: BRD2 non-phospho peptide (aa 31-41).
Exposure time: 10 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
ab223255 has not yet been referenced specifically in any publications.