Overview

  • Product name
    Anti-BRD3 antibody [2088C3a]
    See all BRD3 primary antibodies
  • Description
    Mouse monoclonal [2088C3a] to BRD3
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of BRD3 (Human)

  • Positive control
    • WB: HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and FC: HeLa cells. IP: RIPA lysate of HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB 1/50. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
IP Use at 5-1 µg/mg of lysate.
ICC/IF 1/10.

Target

  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • Sequence similarities
    Contains 2 bromo domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • BRD 3 antibody
      • Brd3 antibody
      • BRD3_HUMAN antibody
      • Bromodomain containing 3 antibody
      • Bromodomain containing protein 3 antibody
      • Bromodomain-containing protein 3 antibody
      • KIAA0043 antibody
      • Open reading frame X antibody
      • ORFX antibody
      • RING3 like gene antibody
      • RING3-like protein antibody
      • RING3L antibody
      see all

    Images

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg

      Secondary
      Anti Mouse IgG antibody at 1/2500 dilution

      Predicted band size: 80 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg

      Secondary
      Anti-Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 80 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

      Secondary
      Anti-Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 80 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    • Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )
    • Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)
      Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)
      Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 µg)
    • Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.

    References

    This product has been referenced in:
    • Kumar K  et al. BET inhibitors block pancreatic stellate cell collagen I production and attenuate fibrosis in vivo. JCI Insight 2:e88032 (2017). Read more (PubMed: 28194432) »

    See 1 Publication for this product

    Publishing research using ab50818? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-BRD3 antibody [2088C3a]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Loading amount
    15 µg
    Specification
    MEFs
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jan 29 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up