Anti-BRD3 antibody [2088C3a] (ab50818)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-BRD3 antibody [2088C3a]
See all BRD3 primary antibodies -
DescriptionMouse monoclonal [2088C3a] to BRD3
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment of BRD3 (Human)
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Positive control
- WB: HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and FC: HeLa cells. IP: RIPA lysate of HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading...
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PurityProtein G purified
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone number2088C3a
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | Use a concentration of 0.5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB | 1/50. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa). | |
IP | Use at 5-1 µg/mg of lysate. | |
ICC/IF | 1/10. |
Target
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Tissue specificityUbiquitous.
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Involvement in diseaseNote=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
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Sequence similaritiesContains 2 bromo domains.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 8019 Human
- Omim: 601541 Human
- SwissProt: Q15059 Human
- Unigene: 522472 Human
- Unigene: 654869 Human
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Alternative names
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
see all
Images
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Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg
Secondary
Anti Mouse IgG antibody at 1/2500 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )
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Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 µg)
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Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Chan KH et al. Impact of Target Warhead and Linkage Vector on Inducing Protein Degradation: Comparison of Bromodomain and Extra-Terminal (BET) Degraders Derived from Triazolodiazepine (JQ1) and Tetrahydroquinoline (I-BET726) BET Inhibitor Scaffolds. J Med Chem 61:504-513 (2018). Read more (PubMed: 28595007) »
- Gadd MS et al. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol 13:514-521 (2017). Read more (PubMed: 28288108) »