Anti-BRD3 antibody [2088C3a] (ab50818)

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Overview

  • Product name
    Anti-BRD3 antibody [2088C3a]
    See all BRD3 primary antibodies
  • Description
    Mouse monoclonal [2088C3a] to BRD3
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of BRD3 (Human)

  • Positive control
    • WB: HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and FC: HeLa cells. IP: RIPA lysate of HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab50818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB 1/50. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
IP Use at 5-1 µg/mg of lysate.
ICC/IF 1/10.

Target

  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • Sequence similarities
    Contains 2 bromo domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • BRD 3 antibody
      • Brd3 antibody
      • BRD3_HUMAN antibody
      • Bromodomain containing 3 antibody
      • Bromodomain containing protein 3 antibody
      • Bromodomain-containing protein 3 antibody
      • KIAA0043 antibody
      • Open reading frame X antibody
      • ORFX antibody
      • RING3 like gene antibody
      • RING3-like protein antibody
      • RING3L antibody
      see all

    Images

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg

      Secondary
      Anti Mouse IgG antibody at 1/2500 dilution

      Predicted band size: 80 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg

      Secondary
      Anti-Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 80 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)
      Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

      Secondary
      Anti-Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 80 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )
    • Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)
      Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)
      Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 µg)
    • Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)
      Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.

    References

    This product has been referenced in:
    • Chan KH  et al. Impact of Target Warhead and Linkage Vector on Inducing Protein Degradation: Comparison of Bromodomain and Extra-Terminal (BET) Degraders Derived from Triazolodiazepine (JQ1) and Tetrahydroquinoline (I-BET726) BET Inhibitor Scaffolds. J Med Chem 61:504-513 (2018). Read more (PubMed: 28595007) »
    • Gadd MS  et al. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol 13:514-521 (2017). Read more (PubMed: 28288108) »
    See all 4 Publications for this product

    Publishing research using ab50818? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-BRD3 antibody [2088C3a]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Loading amount
    15 µg
    Specification
    MEFs
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

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    Verified customer

    Submitted Jan 29 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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