WB: HeLa, F2408 and NIH3T3 whole cell lysates.
ICC/IF and FC: HeLa cells.
IP: RIPA lysate of HeLa cells.
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/50. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
Use at 5-1 µg/mg of lysate.
Involvement in disease
Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.