Product nameAnti-BRD3 antibody
See all BRD3 primary antibodies
DescriptionRabbit polyclonal to BRD3
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1
Database link: Q15059
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 80 kDa.|
Involvement in diseaseNote=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Sequence similaritiesContains 2 bromo domains.
- Information by UniProt
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
All lanes : Anti-BRD3 antibody (ab264294) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 80 kDa
Exposure time: 30 seconds
BRD3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264294 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264294 at 1 µg/ml.
Lane 1: ab264294 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling BRD3 with ab264294 at 1/100 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264294 has not yet been referenced specifically in any publications.