Key features and details
- Rabbit polyclonal to BRD3
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BRD3 antibody
See all BRD3 primary antibodies
DescriptionRabbit polyclonal to BRD3
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- HepG2 whole cell lysate (ab7900).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Involvement in diseaseNote=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Sequence similaritiesContains 2 bromo domains.
- Information by UniProt
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
Anti-BRD3 antibody (ab83478) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 80 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling BRD3 with ab83478 at 10µg/ml.
ab83478 has been referenced in 2 publications.
- Bowry A et al. BET Inhibition Induces HEXIM1- and RAD51-Dependent Conflicts between Transcription and Replication. Cell Rep 25:2061-2069.e4 (2018). PubMed: 30463005
- Sun Y et al. BET protein inhibition mitigates acute myocardial infarction damage in rats via the TLR4/TRAF6/NF-?B pathway. Exp Ther Med 10:2319-2324 (2015). WB . PubMed: 26668635