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  1. Link

    brd4-antibody-epr51502-ab128874.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Acetyl Lysine
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Brd4 antibody [EPR5150(2)] (ab128874)

  • Datasheet
  • SDS
Reviews (17)Q&A (1)References (109)

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Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Flow Cytometry (Intracellular) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • OI-RD Scanning - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
  • Anti-Brd4 antibody [EPR5150(2)] (ab128874)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5150(2)] to Brd4
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-Brd4 antibody [EPR5150(2)]
    See all Brd4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5150(2)] to Brd4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Brd4 aa 150-250. The exact sequence is proprietary.
    Database link: O60885

  • Positive control

    • WB: HeLa, Caco-2, TT, RAW 264.7 and NIH/3T3 cell lysate. Wild-type HAP1 lysate. ICC/IF: HeLa and HepG2 cells. IHC-P: Human colon carcinoma and brain tissue. Flow Cyt (intra): SW480 cells. IP: HEK-239 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 3.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5150(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Acetyl Lysine
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Bromodomain containing
    • Epigenetics and Nuclear Signaling
    • Bromodomains

Associated products

  • Alternative Versions

    • Anti-Brd4 antibody [EPR5150(2)] - BSA and Azide free (ab182446)
    • Alexa Fluor® 488 Anti-Brd4 antibody [EPR5150(2)] (ab197606)
    • Alexa Fluor® 647 Anti-Brd4 antibody [EPR5150(2)] (ab197608)
    • HRP Anti-Brd4 antibody [EPR5150(2)] (ab197609)
    • Alexa Fluor® 594 Anti-Brd4 antibody [EPR5150(2)] (ab207057)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
  • Recombinant Protein

    • Recombinant Human Brd4 protein (Tagged) (ab176941)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.
WB (9)
1/200 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).

 

IHC-P (3)
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/40

See IHC antigen retrieval protocols.

ICC/IF (4)
1/200.

For unpurified use at 1/40

Notes
Flow Cyt (Intra)
1/50.
WB
1/200 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).

 

IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/40

See IHC antigen retrieval protocols.

ICC/IF
1/200.

For unpurified use at 1/40

Target

  • Function

    Plays a role in a process governing chromosomal dynamics during mitosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • Sequence similarities

    Contains 2 bromo domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60885 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23476 Human
    • Entrez Gene: 57261 Mouse
    • Entrez Gene: 362844 Rat
    • Omim: 608749 Human
    • SwissProt: O60885 Human
    • SwissProt: Q9ESU6 Mouse
    • Unigene: 187763 Human
    • Unigene: 253518 Mouse
    • Alternative names

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    Images

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Brd4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab128874 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab128874 was shown to recognize Brd4 when Brd4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Brd4 knockout samples were subjected to SDS-PAGE. ab128874 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)Ren et al PLoS Pathog. 2016 Oct; 12(10): e1005950. Published online 2016 Oct 20. doi: 10.1371/journal.ppat.1005950

      Immunocytochemical analysis of HeLa cells showing co-localization of BRD4 using ab128874 at 1/500 dilution (red) with CDK9 (green) following infection with HSV-1. Cells were fixed with 4% paraformaldehyde (10 min at RT) and permeabilzed with 0.2% Triton X-100 (10 min). AlexaFluor®-conjugated secondary antibodies were used at 1/1000 dilution. The nuclear counter stain is DAPI 9blue).

      From Figure 5b of Ren et al.

      Reproduced under the Creative Commons Licence from Ren et al PLoS Pathog. 2016 Oct; 12(10): e1005950. Published online 2016 Oct 20. doi: 10.1371/journal.ppat.1005950 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)

      Unpurified ab128874 at 1/100 dilution staining Brd4 in human colon carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Flow Cytometry (Intracellular) - Anti-Brd4 antibody [EPR5150(2)] (ab128874)

      Intracellular Flow Cytometry analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling Brd4 (red) with purified ab128874 at a 1/50 dilution (10µg/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluorr®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      All lanes : Anti-Brd4 antibody [EPR5150(2)] (ab128874) at 1/200 dilution

      Lane 1 : Wild-type HAP1 cell lysate
      Lane 2 : BRD4 knockout HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 152 kDa
      Observed band size: 220 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab128874 observed at 220 kDa. Red - loading control Mouse anti Vinculin observed at 125 kDa.

      ab128874 was shown to react with Brd4 in wild-type cells in Western blot with loss of signal observed in BRD4 knockout sample. Wild-type and BRD4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab128874 and Mouse anti Vinculin overnight at 4 °C at a 1 in 200 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver cell line) cells labeling Brd4 with purified ab128874 at 1/100 (Panel A). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain(Panel B).

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Anti-Brd4 antibody [EPR5150(2)] (ab128874) at 1/1000 dilution (Purified) + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 152 kDa
      Observed band size: 152 kDa

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Different batches of ab128874 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 152 kDa.
    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)] (ab128874)Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

      Unpurified ab128874 (1/500) staining Brd4 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red).

      For further experimental details please refer to Abreview.

      See Abreview

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Anti-Brd4 antibody [EPR5150(2)] (ab128874) at 1/1000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 152 kDa
      Observed band size: 152 kDa



      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Western blot - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Anti-Brd4 antibody [EPR5150(2)] (ab128874) at 1/200 dilution (unpurified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg/ml

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 152 kDa
      Observed band size: 152 kDa



      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    • OI-RD Scanning - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      OI-RD Scanning - Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Brd4 antibody [EPR5150(2)] (ab128874)
      Anti-Brd4 antibody [EPR5150(2)] (ab128874)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (109)

    Publishing research using ab128874? Please let us know so that we can cite the reference in this datasheet.

    ab128874 has been referenced in 109 publications.

    • Latif AL  et al. BRD4-mediated repression of p53 is a target for combination therapy in AML. Nat Commun 12:241 (2021). PubMed: 33431824
    • Kondo H  et al. Single-cell resolved imaging reveals intra-tumor heterogeneity in glycolysis, transitions between metabolic states, and their regulatory mechanisms. Cell Rep 34:108750 (2021). PubMed: 33596424
    • Zhao P  et al. LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis. Cancer Cell Int 21:14 (2021). PubMed: 33407504
    • He Y  et al. A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Nat Commun 12:1521 (2021). PubMed: 33750801
    • Sheppard HE  et al. Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity. Cell Rep Med 2:100188 (2021). PubMed: 33521702
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-10 of 17 Abreviews

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 09 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Brd4 antibody [EPR5150(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 08 2021

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Umbilical Vein Endothelial cell)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    24 µg
    Treatment
    Doxorubicin 16nM 24hrs
    Specification
    Human Umbilical Vein Endothelial cell
    Blocking step
    Sea Block Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
    Read More

    Shixin Tao

    Verified customer

    Submitted Oct 28 2021

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    25 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    DR. Robert Kupp

    Verified customer

    Submitted Oct 19 2020

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular Smooth Muscle Cells)
    Permeabilization
    Yes - NP-40
    Specification
    Vascular Smooth Muscle Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    fibroblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2018

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Brd4 antibody [EPR5150(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 20 2017

    Western blot abreview for Anti-Brd4 antibody [EPR5150(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Sample
    Mouse Cell lysate - whole cell (pancreatic cancer)
    Specification
    pancreatic cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    MS. Jamie Plevy

    Verified customer

    Submitted Sep 26 2014

    1-10 of 17 Abreviews

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