Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5150(2)] to Brd4 (Alexa Fluor® 488)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Product nameAnti-Brd4 antibody [EPR5150(2)] (Alexa Fluor® 488)
See all Brd4 primary antibodies
DescriptionRabbit monoclonal [EPR5150(2)] to Brd4 (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Brd4 aa 150-250. The exact sequence is proprietary.
- ICC/IF: SW480 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 3.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Brd4 antibody [EPR5150(2)] (ab128874)
- Anti-Brd4 antibody [EPR5150(2)] - BSA and Azide free (ab182446)
- Anti-Brd4 antibody [EPR5150(2)] (Alexa Fluor® 647) (ab197608)
- Anti-Brd4 antibody [EPR5150(2)] (HRP) (ab197609)
- Anti-Brd4 antibody [EPR5150(2)] (Alexa Fluor® 594) (ab207057)
- Anti-Brd4 antibody [EPR5150(2)] (Alexa Fluor® 568) (ab207060)
Our Abpromise guarantee covers the use of ab197606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in SW480 cells fixed with 100% methanol (5 min).
FunctionPlays a role in a process governing chromosomal dynamics during mitosis.
Tissue specificityUbiquitously expressed.
Involvement in diseaseNote=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
Sequence similaritiesContains 2 bromo domains.
- Information by UniProt
- Brd4 antibody
- BRD4-NUT FUSION antibody
- BRD4-NUT fusion oncoprotein antibody
ab197606 staining Brd4 in SW480 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197606 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab197606 has not yet been referenced specifically in any publications.