Product nameAnti-BRD7 antibody
See all BRD7 primary antibodies
DescriptionRabbit polyclonal to BRD7
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human BRD7 aa 601-651. The exact sequence is proprietary. NP_037395.2
Database link: Q9NPI1
- WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human prostate carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245599 was affinity purified using an epitope specific to BRD7 immobilized on solid support.
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab245599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 74 kDa.|
FunctionActs both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Sequence similaritiesContains 1 bromo domain.
- Information by UniProt
- 75 kDa bromodomain protein antibody
- BP75 antibody
- BRD 7 antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for BRD7 using ab245599 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-BRD7 antibody (ab245599) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
ab245599 has not yet been referenced specifically in any publications.