Overview

  • Product name

  • Description

    Rabbit polyclonal to BRD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human BRD7 aa 601-651. The exact sequence is proprietary. NP_037395.2
    Database link: Q9NPI1

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human prostate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
    • Sequence similarities

      Contains 1 bromo domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 75 kDa bromodomain protein antibody
      • BP75 antibody
      • BRD 7 antibody
      • BRD7 antibody
      • BRD7_HUMAN antibody
      • Bromodomain containing 7 antibody
      • bromodomain containing protein 7 antibody
      • Bromodomain-containing protein 7 antibody
      • CELTIX 1 antibody
      • CELTIX1 antibody
      • NAG4 antibody
      • Protein CELTIX-1 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for BRD7 using ab245599 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • All lanes : Anti-BRD7 antibody (ab245599) at 0.4 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 74 kDa


      Exposure time: 3 minutes

    References

    ab245599 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245599.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up