Overview

  • Product name
  • Description
    Rabbit polyclonal to BRD7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Cow, Dog, Zebrafish, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human BRD7.

    Read Abcam's proprietary immunogen policy (Peptide available as ab73296.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, SW480, SHSY-5Y, HeLa - Hydroxyurea Treated (48hr, 2µM) (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab46553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

Target

  • Function
    Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
  • Sequence similarities
    Contains 1 bromo domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 75 kDa bromodomain protein antibody
    • BP75 antibody
    • BRD 7 antibody
    • BRD7 antibody
    • BRD7_HUMAN antibody
    • Bromodomain containing 7 antibody
    • bromodomain containing protein 7 antibody
    • Bromodomain-containing protein 7 antibody
    • CELTIX 1 antibody
    • CELTIX1 antibody
    • NAG4 antibody
    • Protein CELTIX-1 antibody
    see all

Images

  • All lanes : Anti-BRD7 antibody (ab46553) at 1/250 dilution

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : SW480 whole cell lysate (ab3957)
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa
    Additional bands at: 150 kDa, 16 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

ab46553 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

These two antibodies (ab46553, ab56036) you mentioned are polyclonal antibodies. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Generally, epitope mapping experiments for polyclonal antibodies are not performed - only for monoclonal antibodies.

Regarding the immunogen sequence, if it is not commercially sensitive information, we can publish it on-line on the product datasheets. Unfortunately, we are unable to release proprietary information and this is the case for ab56036.

ab46553 and ab56036 are from two different sources and their manufacture process, purification and characterization (tested applications) are different as well. The prices of our antibodies reflect all of these factors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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