Product nameAnti-BRD7 antibody
See all BRD7 primary antibodies
DescriptionRabbit polyclonal to BRD7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken, Cow, Dog, Zebrafish, Rhesus monkey, Orangutan
- This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, SW480, SHSY-5Y, HeLa - Hydroxyurea Treated (48hr, 2µM) (data not shown)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab46553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
FunctionActs both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Sequence similaritiesContains 1 bromo domain.
- Information by UniProt
- 75 kDa bromodomain protein antibody
- BP75 antibody
- BRD 7 antibody
All lanes : Anti-BRD7 antibody (ab46553) at 1/250 dilution
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 :
SW480 whole cell lysate (ab3957)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 150 kDa, 16 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab46553 has not yet been referenced specifically in any publications.