Product nameAnti-BRD7 antibody
See all BRD7 primary antibodies
DescriptionRabbit polyclonal to BRD7
Tested applicationsSuitable for: ChIP, ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Guinea pig, Cat, Dog
- Jurkat cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein G purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
ChIP Related Products
Our Abpromise guarantee covers the use of ab56036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20215511|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.2 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.|
FunctionActs both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Sequence similaritiesContains 1 bromo domain.
- Information by UniProt
- 75 kDa bromodomain protein antibody
- BP75 antibody
- BRD 7 antibody
Anti-BRD7 antibody (ab56036) at 0.2 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 56,74 kDa why is the actual band size different from the predicted?
ICC/IF image of ab56036 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56036, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-BRD7 antibody (ab56036) at 2.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size: 74 kDa
This product has been referenced in:
- Cabot B et al. Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. Mol Reprod Dev 84:1238-1249 (2017). ICC/IF . Read more (PubMed: 29024220) »
- Harte MT et al. BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Cancer Res 70:2538-47 (2010). WB, ChIP ; Human . Read more (PubMed: 20215511) »