Anti-BRD8/p120 antibody (ab17968)
Key features and details
- Rabbit polyclonal to BRD8/p120
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRD8/p120 antibody
See all BRD8/p120 primary antibodies -
Description
Rabbit polyclonal to BRD8/p120 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human BRD8/p120 aa 1-100.
Database link: Q9H0E9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/25000. Predicted molecular weight: 120 kDa.
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Notes |
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WB
1/10000 - 1/25000. Predicted molecular weight: 120 kDa. |
Target
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Function
May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. -
Tissue specificity
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 416219 Chicken
- Entrez Gene: 10902 Human
- Entrez Gene: 291691 Rat
- Omim: 602848 Human
- SwissProt: Q9H0E9 Human
- SwissProt: Q5TLG7 Rat
- Unigene: 519337 Human
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Alternative names
- Brd8 antibody
- BRD8_HUMAN antibody
- Bromodomain containing 8 antibody
see all
Images
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Ab17968 at the concentrations stated, staining human BRD8/p120 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.
Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab17968 has been referenced in 1 publication.
- Kim J et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . PubMed: 20946988