Key features and details
- Rabbit polyclonal to BRD8/p120
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BRD8/p120 antibody
See all BRD8/p120 primary antibodies
DescriptionRabbit polyclonal to BRD8/p120
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Previously labelled as BRD8
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab17968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/25000. Predicted molecular weight: 120 kDa.|
|IP||Use 2-8µg for 108 cells.|
RelevanceBRD8 / p120 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.
- BRD8 antibody
- Bromodomain containing 8 antibody
- p120 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8/p120 with ab17968 at 1/1000 (1µg/ml). Detection: DAB.
Ab17968 at the concentrations stated, staining human BRD8/p120 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.
Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.
ab17968 has been referenced in 1 publication.
- Kim J et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . PubMed: 20946988