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    brd8p120-antibody-ab17969.pdf

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Cell Biology Apoptosis Nucleus Other
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Anti-BRD8/p120 antibody (ab17969)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)
  • Western blot - Anti-BRD8/p120 antibody (ab17969)

Key features and details

  • Rabbit polyclonal to BRD8/p120
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BRD8/p120 antibody
    See all BRD8/p120 primary antibodies
  • Description

    Rabbit polyclonal to BRD8/p120
  • Host species

    Rabbit
  • Specificity

    Specific to p120
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide within BRD8/p120 aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9H0E9

  • Positive control

    • Nuclear extract from HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Bromodomain containing
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Epigenetics and Nuclear Signaling
    • Bromodomains

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
Notes
IP
Use at an assay dependent concentration.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).

Target

  • Function

    May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.
  • Tissue specificity

    Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Sequence similarities

    Contains 2 bromo domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9H0E9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10902 Human
    • Entrez Gene: 291691 Rat
    • Omim: 602848 Human
    • SwissProt: Q9H0E9 Human
    • SwissProt: Q5TLG7 Rat
    • Unigene: 519337 Human
    • Alternative names

      • Brd8 antibody
      • BRD8_HUMAN antibody
      • Bromodomain containing 8 antibody
      • Bromodomain-containing protein 8 antibody
      • p120 antibody
      • Skeletal muscle abundant protein 2 antibody
      • Skeletal muscle abundant protein antibody
      • SMAP antibody
      • SMAP2 antibody
      • Thyroid hormone receptor coactivating protein 120kDa antibody
      • Thyroid hormone receptor coactivating protein antibody
      • Thyroid hormone receptor coactivating protein of 120 kDa antibody
      • TrCP120 antibody
      see all

    Images

    • Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
      Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)

      ab17969 immunoprecipitating BRD8 at 6 µg per reaction.

      Lane 1: ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).

      Lane 2: IgG control

      For blotting immunoprecipitated BRD8, ab17969 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.

    • Western blot - Anti-BRD8/p120 antibody (ab17969)
      Western blot - Anti-BRD8/p120 antibody (ab17969)
      Detection of human BRD8 by Western Blot. Sample is nuclear
      extract (NE; 5, 15, and 50mcg) from HeLa cells.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab17969? Please let us know so that we can cite the reference in this datasheet.

    ab17969 has been referenced in 2 publications.

    • Couture JP  et al. The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPAR? target genes. Endocrinology 153:5796-808 (2012). PubMed: 23064015
    • Yamaguchi K  et al. C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer. Br J Cancer 102:325-31 (2010). WB, IP ; Human . PubMed: 20051959

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I just have a question regarding the antibody that I purchased (Anti-BRD8 antibody ab17969): would you please tell me if it recognizes the isoform 1 (920 aa) or isoform 2 (1235 aa) of BRD8. Since in the sheet it is written that the epitope maps to a region between amino acid 200/250 of human BRD8, and in fact this is a region differs between two isoforms. Although it is also written that the antibody is specific to p120 but I am a bit confused about this name in the databases, and wonder which isoform is indeed p120!!!
    I would greatly appreciate for your consideration and help.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 22 2013

    Answer



    There has been a revision in the entry for this target on the swissprot home page.

    The epitope recognized by ab17969 is found within isoform 2 according to the current version of Uniprot entry Q9H0E9.

    http://www.uniprot.org/uniprot/Q9H0E9

    More specifically, the epitope is found within entry Q9H0E9.2 which is isoform 2 and also known as p120 (102 kDa calculated size plus the modifications).

    Read More

    Abcam Scientific Support

    Answered on Apr 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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