Anti-BRD8/p120 antibody (ab17969)
Key features and details
- Rabbit polyclonal to BRD8/p120
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRD8/p120 antibody
See all BRD8/p120 primary antibodies -
Description
Rabbit polyclonal to BRD8/p120 -
Host species
Rabbit -
Specificity
Specific to p120 -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant
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Immunogen
Synthetic peptide within BRD8/p120 aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9H0E9 -
Positive control
- Nuclear extract from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa). |
Target
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Function
May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. -
Tissue specificity
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10902 Human
- Entrez Gene: 291691 Rat
- Omim: 602848 Human
- SwissProt: Q9H0E9 Human
- SwissProt: Q5TLG7 Rat
- Unigene: 519337 Human
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Alternative names
- Brd8 antibody
- BRD8_HUMAN antibody
- Bromodomain containing 8 antibody
see all
Images
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Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
ab17969 immunoprecipitating BRD8 at 6 µg per reaction.
Lane 1: ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).
Lane 2: IgG control
For blotting immunoprecipitated BRD8, ab17969 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-BRD8/p120 antibody (ab17969)Detection of human BRD8 by Western Blot. Sample is nuclear
extract (NE; 5, 15, and 50mcg) from HeLa cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab17969 has been referenced in 2 publications.
- Couture JP et al. The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPAR? target genes. Endocrinology 153:5796-808 (2012). PubMed: 23064015
- Yamaguchi K et al. C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer. Br J Cancer 102:325-31 (2010). WB, IP ; Human . PubMed: 20051959