Anti-BRD8/p120 antibody (ab17969)

Submit a review Q&A (1)References (2)

Key features and details

  • Rabbit polyclonal to BRD8/p120
  • Suitable for: ICC/IF, IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-BRD8/p120 antibody
    See all BRD8/p120 primary antibodies

  • Description

    Rabbit polyclonal to BRD8/p120

  • Host species

    Rabbit

  • Specificity

    Specific to p120

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant

  • Immunogen

    Synthetic peptide within BRD8/p120 aa 225-275. The exact sequence is proprietary.

  • Positive control

    • Nuclear extract from HeLa cells.

  • General notes

    Previously labelled as BRD8

Properties

Applications

Our Abpromise guarantee covers the use of ab17969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
IP Use at 2-8 µg/mg of lysate.

Target

  • Relevance

    BRD8 / p120 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • BRD8 antibody
      • Bromodomain containing 8 antibody
      • p120 antibody
      • Skeletal muscle abundant protein antibody
      • Skeletal muscle abundant protein 2 antibody
      • SMAP antibody
      • SMAP2 antibody
      • Thyroid hormone receptor coactivating protein 120kDa antibody
      • Thyroid hormone receptor coactivating protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.

    • Western blot - Anti-BRD8/p120 antibody (ab17969)
      Western blot - Anti-BRD8/p120 antibody (ab17969)
      Detection of human BRD8 by Western Blot. Sample is nuclear
      extract (NE; 5, 15, and 50mcg) from HeLa cells.
    • Immunocytochemistry/ Immunofluorescence - Anti-BRD8/p120 antibody (ab17969)
      Immunocytochemistry/ Immunofluorescence - Anti-BRD8/p120 antibody (ab17969)
      ICC/IF image of ab17969 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    Publishing research using ab17969? Please let us know so that we can cite the reference in this datasheet.

    ab17969 has been referenced in 2 publications.

    • Couture JP  et al. The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPAR? target genes. Endocrinology 153:5796-808 (2012). PubMed: 23064015
    • Yamaguchi K  et al. C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer. Br J Cancer 102:325-31 (2010). WB, IP ; Human . PubMed: 20051959

    Customer reviews and Q&As

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    Question

    I just have a question regarding the antibody that I purchased (Anti-BRD8 antibody ab17969): would you please tell me if it recognizes the isoform 1 (920 aa) or isoform 2 (1235 aa) of BRD8. Since in the sheet it is written that the epitope maps to a region between amino acid 200/250 of human BRD8, and in fact this is a region differs between two isoforms. Although it is also written that the antibody is specific to p120 but I am a bit confused about this name in the databases, and wonder which isoform is indeed p120!!!
    I would greatly appreciate for your consideration and help.
    Best regards,

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    Answer



    There has been a revision in the entry for this target on the swissprot home page.

    The epitope recognized by ab17969 is found within isoform 2 according to the current version of Uniprot entry Q9H0E9.

    http://www.uniprot.org/uniprot/Q9H0E9

    More specifically, the epitope is found within entry Q9H0E9.2 which is isoform 2 and also known as p120 (102 kDa calculated size plus the modifications).

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