Overview

  • Product name
  • Description
    Rabbit polyclonal to BRD9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 547-597 of Human BRD9 (NP_076413.3).

  • Positive control
    • Jurkat, 293T and HeLa whole cell lysate (ab150035)

Properties

Applications

Our Abpromise guarantee covers the use of ab137245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    BRD9 is a bromodomain containing protein, which are known to bind to acetylated lysine residues.
  • Database links
  • Alternative names
    • Bromodomain containing 9 antibody
    • Bromodomain containing protein 9 antibody
    • DKFZp434D0711 antibody
    • DKFZp686L0539 antibody
    • FLJ13441 antibody
    • LAVS3040 antibody
    • PRO9856 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.8 antibody
    • Sarcoma antigen NY SAR 29 antibody
    • UNQ3040 antibody
    see all

Images

  • All lanes : Anti-BRD9 antibody (ab137245) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation with ab137245 at 6µg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Western blot labelling BRD9 with ab137245 at 1 µg/ml. Detected by chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:
  • Pan J  et al. Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Syst 6:555-568.e7 (2018). Read more (PubMed: 29778836) »
  • Hohmann AF  et al. Sensitivity and engineered resistance of myeloid leukemia cells to BRD9 inhibition. Nat Chem Biol 12:672-9 (2016). Read more (PubMed: 27376689) »
See all 2 Publications for this product

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