Recombinant Anti-BRDT antibody [EPR25427-24] (ab288435)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25427-24] to BRDT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-BRDT antibody [EPR25427-24]
See all BRDT primary antibodies -
Description
Rabbit monoclonal [EPR25427-24] to BRDT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse testis and Rat testis IHC-P: Mouse testis and Rat testis
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25427-24 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 108 kDa.
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IHC-P |
1/2000.
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Notes |
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WB
1/1000. Predicted molecular weight: 108 kDa. |
IHC-P
1/2000. |
Target
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Function
May play a role in the transcriptional regulation of spermatogenesis. Seems to have a structural ATP-independent role in the reorganization of acetylated chromatin. -
Tissue specificity
Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder. -
Sequence similarities
Contains 2 bromo domains. -
Developmental stage
Expressed in embryo testis. -
Domain
The two bromodomains are involved in the specific recognition of histone H4 acetylated N-terminus. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 114642 Mouse
- Entrez Gene: 305123 Rat
- SwissProt: Q91Y44 Mouse
- SwissProt: D4A7T3 Rat
- Unigene: 182836 Mouse
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Alternative names
- BRD 6 antibody
- BRD6 antibody
- Brdt antibody
see all
Images
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All lanes : Anti-BRDT antibody [EPR25427-24] (ab288435) at 1/1000 dilution
Lane 1 : Human BRDT recombinant protein (aa 1-947) 20ng
Lane 2 : Human BRD3 recombinant protein (aa 1-726) 20ng
Lane 3 : Human BRD4 recombinant protein (aa 49-240) 20ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 59 seconds
This antibody has no cross-reaction with BRD3 and BRD4.
All the recombinant proteins are expressed from an E.coli expression system. -
All lanes : Anti-BRDT antibody [EPR25427-24] (ab288435) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate at 20 µg
Lane 2 : Mouse kidney tissue lysate at 20 µg
Lane 3 : Mouse skeletal muscle tissue lysate at 20 µg
Lane 4 : Rat testis tissue lysate at 20 µg
Lane 5 : Rat kidney tissue lysate at 40 µg
Lane 6 : Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 125 seconds
Low expression: Kidney, Skeletal muscle
The observed MW/expression profile is consistent with what has been described in the literature (PMID: 33257688 & Uniprot) -
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Nuclear staining on germ cells in mouse testis. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Nuclear staining on germ cells in rat testis. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on rat skeletal muscle. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on mouse kidney. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab288435 has not yet been referenced specifically in any publications.