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BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

  • Datasheet
  • SDS
  • COA
Reviews (1) Submit a question References (23)

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Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
  • Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

Key features and details

  • Thymidine analog for measuring cell proliferation
  • CAS Number: 59-14-3
  • Purity: > 98%
  • Soluble in water to 50 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
  • Description

    Thymidine analog for measuring cell proliferation
  • Purity

    > 98%
  • General notes

    Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956. 

  • CAS Number

    59-14-3
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
  • Molecular weight

    307.10
  • Molecular formula

    C9H11BrN2O5
  • PubChem identifier

    6035
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    BrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2
  • Source

    Synthetic

  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Biochemicals
    • Product Range
    • Just Add Water
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Chemical Type
    • Nucleoside
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Fluorescent dyes and reagents
    • Biochemicals
    • Pharmacology
    • Signaling
    • DNA, RNA & protein synthesis
    • DNA & RNA synthesis
    • Biochemicals
    • Chemical Type
    • Dyes, stains and fluorescent tools

Associated products

  • Related Products

    • BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    • BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Images

  • Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
  • Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.

    (From Figure 5A of Massey)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (23)

Publishing research using ab142567? Please let us know so that we can cite the reference in this datasheet.

ab142567 has been referenced in 23 publications.

  • Xu P  et al. PAQR4 promotes chemoresistance in non-small cell lung cancer through inhibiting Nrf2 protein degradation. Theranostics 10:3767-3778 (2020). PubMed: 32206121
  • Cao Y  et al. N-Acetyltransferase 10 Promotes Micronuclei Formation to Activate the Senescence-Associated Secretory Phenotype Machinery in Colorectal Cancer Cells. Transl Oncol 13:100783 (2020). PubMed: 32428852
  • Zietzer A  et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles 9:1786967 (2020). PubMed: 32944175
  • Yang HJ  et al. Establishment and Characterization of Immortalized Miniature Pig Pancreatic Cell Lines Expressing Oncogenic K-RasG12D. Int J Mol Sci 21:N/A (2020). PubMed: 33233448
  • Hilgendorf KI  et al. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis. Cell 179:1289-1305.e21 (2019). PubMed: 31761534
View all Publications for this product

Customer reviews and Q&As

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It works very well

Excellent
Abreviews
Abreviews
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It works very well. The complementary protocol on the Abcam website is straightforward and easy to follow.
I used it at the concentration of 10 micromolar and left for 2 hours in culture.

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Verified customer

Submitted Nov 27 2017

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