BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Key features and details
- Thymidine analog for measuring cell proliferation
- CAS Number: 59-14-3
- Purity: > 98%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
BrdU (5-bromo-2'-deoxyuridine), Thymidine analog -
Description
Thymidine analog for measuring cell proliferation -
Purity
> 98% -
General notes
Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
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CAS Number
59-14-3 -
Chemical structure
Properties
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Chemical name
5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione -
Molecular weight
307.10 -
Molecular formula
C9H11BrN2O5 -
PubChem identifier
6035 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
BrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2 -
Source
Synthetic
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Research areas
Associated products
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Related Products
Images
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Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
ab220075 staining BrdU in HeLa cells. Untreated and BrdU treated (ab142567, 10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab220075 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
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Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog -
Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.
(From Figure 5A of Massey)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (37)
ab142567 has been referenced in 37 publications.
- Pavel M et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
- Bilotta AJ et al. Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair. Cell Mol Gastroenterol Hepatol 11:1023-1044 (2021). PubMed: 33238220
- Cui S & Li C RHPN1-AS1 promotes ovarian carcinogenesis by sponging miR-485-5p and releasing TPX2 mRNA. Oncol Rep 45:N/A (2021). PubMed: 33907841
- Sobel J et al. Scrt1, a transcriptional regulator of ß-cell proliferation identified by differential chromatin accessibility during islet maturation. Sci Rep 11:8800 (2021). PubMed: 33888791
- Cao JY et al. Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury. Theranostics 11:5248-5266 (2021). PubMed: 33859745