BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
- Datasheet
- References (9)
Overview
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Product nameBrdU (5-bromo-2'-deoxyuridine), Thymidine analog
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DescriptionThymidine analog for measuring cell proliferation
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Biological description
Thymidine analog, naturally incorporates into proliferating cells. Labels DNA, useful marker for study of DNA synthesis. Decreases TTP pools in vitro. Antitumor and radiation-sensitizing agent. Active in vitro and in vivo.
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Purity> 98%
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General notes
Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
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CAS Number59-14-3
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Chemical structure
Properties
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Chemical name5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
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Molecular weight307.10
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Molecular formulaC9H11BrN2O5
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PubChem identifier6035
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Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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Solubility overviewSoluble in water to 50 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESBrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2
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Source
Synthetic
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Research areas
Associated products
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Related Products
Images
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Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015.Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.
(From Figure 5A of Massey)
References
This product has been referenced in:
- Takagi S et al. Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment. Cytometry 14:640-8 (1993). Read more (PubMed: 8404370) »
- Shewach DS et al. Decrease in TTP pools mediated by 5-bromo-2'-deoxyuridine exposure in a human glioblastoma cell line. Biochem Pharmacol 43:1579-85 (1992). Read more (PubMed: 1567479) »
- Brennan LM et al. An in vivo method for using 5-bromo-2'-deoxyuridine (BrdU) as a marker of chemically-induced hepatocellular proliferation in the Japanese medaka (Oryzias latipes). Toxicol Pathol 29:387-97 (0). Read more (PubMed: 11442025) »
