BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Overview
-
Product name
BrdU (5-bromo-2'-deoxyuridine), Thymidine analog -
Description
Thymidine analog for measuring cell proliferation -
Biological description
Thymidine analog, naturally incorporates into proliferating cells. Labels DNA, useful marker for study of DNA synthesis. Decreases TTP pools in vitro. Antitumor and radiation-sensitizing agent. Active in vitro and in vivo.
-
Purity
> 98% -
General notes
Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
-
CAS Number
59-14-3 -
Chemical structure
Properties
-
Chemical name
5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione -
Molecular weight
307.10 -
Molecular formula
C9H11BrN2O5 -
PubChem identifier
6035 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
BrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2 -
Source
Synthetic
-
Research areas
Associated products
-
Related Products
Images
-
Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.
(From Figure 5A of Massey)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab142567 has been referenced in 9 publications.
- Jiang LP et al. GRK5 functions as an oncogenic factor in non-small-cell lung cancer. Cell Death Dis 9:295 (2018). PubMed: 29463786
- Yang CP et al. Comprehensive integrative analyses identify GLT8D1 and CSNK2B as schizophrenia risk genes. Nat Commun 9:838 (2018). PubMed: 29483533
- Kim BH et al. Synergistic actions of FGF2 and bone marrow transplantation mitigate radiation-induced intestinal injury. Cell Death Dis 9:383 (2018). PubMed: 29515101
- Mukai T et al. Umbilical Cord-Derived Mesenchymal Stromal Cells Contribute to Neuroprotection in Neonatal Cortical Neurons Damaged by Oxygen-Glucose Deprivation. Front Neurol 9:466 (2018). PubMed: 29963009
- Massey AJ Multiparametric Cell Cycle Analysis Using the Operetta High-Content Imager and Harmony Software with PhenoLOGIC. PLoS One 10:e0134306 (2015). PubMed: 26218638
- Gurt I et al. The Sirt1 Activators SRT2183 and SRT3025 Inhibit RANKL-Induced Osteoclastogenesis in Bone Marrow-Derived Macrophages and Down-Regulate Sirt3 in Sirt1 Null Cells. PLoS One 10:e0134391 (2015). PubMed: 26226624
- Thoppil RJ et al. TRPV4 channel activation selectively inhibits tumor endothelial cell proliferation. Sci Rep 5:14257 (2015). PubMed: 26388427
- Bibliowicz J & Gross JM Ectopic proliferation contributes to retinal dysplasia in the juvenile zebrafish patched2 mutant eye. Invest Ophthalmol Vis Sci 52:8868-77 (2011). PubMed: 22003118
- Chauvigné F et al. Time- and dose-related effects of di-(2-ethylhexyl) phthalate and its main metabolites on the function of the rat fetal testis in vitro. Environ Health Perspect 117:515-21 (2009). PubMed: 19440488
