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Anti-BrdU antibody (ab152095)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (28)

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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
  • Flow Cytometry - Anti-BrdU antibody (ab152095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)
  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)

Key features and details

  • Rabbit polyclonal to BrdU
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-BrdU antibody
    See all BrdU primary antibodies
  • Description

    Rabbit polyclonal to BrdU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to BrdU.

  • Positive control

    • ICC/IF: HeLa cells treated with 100M BrdU for 4hrs. IHC-P: mouse BrdU treated intestine Flow Cyt: HeLa treated with 10 M BrdU for 30 minutes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab152095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
Flow Cyt
1/50 - 1/200.
IHC-P (1)
1/100 - 1/1000.
Notes
ICC/IF
1/100 - 1/1000.
Flow Cyt
1/50 - 1/200.
IHC-P
1/100 - 1/1000.

Target

  • Relevance

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization

    Nuclear
  • Alternative names

    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)

    HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.

  • Flow Cytometry - Anti-BrdU antibody (ab152095)
    Flow Cytometry - Anti-BrdU antibody (ab152095)

    ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4%  paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
    Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (28)

    Publishing research using ab152095? Please let us know so that we can cite the reference in this datasheet.

    ab152095 has been referenced in 28 publications.

    • Wu MH  et al. Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation. Chin Med 15:100 (2020). PubMed: 32983251
    • Liu SH  et al. EGFR monoclonal antibody panitumumab inhibits chronic proliferative cholangitis by downregulating EGFR. Int J Mol Med 44:79-88 (2019). PubMed: 31115490
    • Bai Y  et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. Mol Cell 75:1299-1314.e6 (2019). PubMed: 31353207
    • Liu W  et al. Whole blueberry protects pancreatic beta-cells in diet-induced obese mouse. Nutr Metab (Lond) 16:34 (2019). PubMed: 31139236
    • Cui B  et al. Repair of Adult Mammalian Heart After Damages by Oral Intake of Gu Ben Pei Yuan San. Front Physiol 10:607 (2019). PubMed: 31191336
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Resin sections) abreview for Anti-BrdU antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Rabbit Tissue sections (Brain (agar sections))
    Specification
    Brain (agar sections)
    Read More

    Dr. Patricia Sampedro-Piquero

    Verified customer

    Submitted Jan 09 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - 0,25% Triton X-100
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Read More

    Mr. Manuel Narvaez

    Verified customer

    Submitted Oct 16 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse brain)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    mouse brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Jacob Garza

    Verified customer

    Submitted Sep 14 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-BrdU antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.3 % triton
    Fixative
    Paraformaldehyde
    Read More

    Dr. Sébastien Brot

    Verified customer

    Submitted Oct 29 2013

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