Anti-BrdU antibody (ab152095)
- Datasheet
- References (24)
- Protocols
Overview
-
Product name
Anti-BrdU antibody
See all BrdU primary antibodies -
Description
Rabbit polyclonal to BrdU -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU.
-
Positive control
- ICC/IF: HeLa cells treated with 100µM BrdU for 4hrs. IHC-P: mouse BrdU treated intestine FC: HeLa treated with 10 µM BrdU for 30 minutes
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab152095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| Flow Cyt | 1/50 - 1/200. | |
| IHC-P | 1/100 - 1/1000. |
Target
-
Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.
-
Flow Cytometry - Anti-BrdU antibody (ab152095)ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min -
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.
References
This product has been referenced in:
- Seth B et al. Carbofuran hampers oligodendrocytes development leading to impaired myelination in the hippocampus of rat brain. Neurotoxicology 70:161-179 (2019). Read more (PubMed: 30471306) »
- Tang X et al. The role of the fat mass and obesity-associated protein in the proliferation of pancreatic cancer cells. Oncol Lett 17:2473-2478 (2019). Read more (PubMed: 30719115) »
