Anti-BrdU antibody (ab152095)
- Datasheet
- References (14)
- Protocols
Overview
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Product nameAnti-BrdU antibody
See all BrdU primary antibodies -
DescriptionRabbit polyclonal to BrdU
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Immunogen
Chemical/ Small Molecule corresponding to BrdU.
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Positive control
- HeLa cells treated with 100µM BrdU for 4hrs.
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General notes
This antibody has so far been only tested in cultured cells (ICC/IF), but not with tissue sections (IHC).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab152095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 28397811 |
Target
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RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
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Cellular localizationNuclear
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Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.
References
This product has been referenced in:
- Zhang W et al. Lipid Supplement in the Cultural Condition Facilitates the Porcine iPSC Derivation through cAMP/PKA/CREB Signal Pathway. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29419748) »
- Shu T et al. Melatonin promotes neuroprotection of induced pluripotent stem cells-derived neural stem cells subjected to H2O2-induced injury in vitro. Eur J Pharmacol 825:143-150 (2018). Read more (PubMed: 29462594) »
